ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2007
Liquid chromatography mass spectrometry profiling of histones.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Structural and epitope characterization of anti-DEFA5 monoclonal antibodies clones 1A8 and 4F5 for inflammatory bowel disease subtype diagnostics.International journal of biological macromolecules · 2025Article
- Article
- Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications.Epigenetics · 2021Article
- Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS.Journal of proteome research · 2018Article
- Chromatographic efficiency and selectivity in top-down proteomics of histones.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2017Article
- Histone H1 phosphorylation in breast cancer.Journal of proteome research · 2014Article
- H1 histones: current perspectives and challenges.Nucleic acids research · 2013Review
- Isolation and analysis of linker histones across cellular compartments.Journal of proteomics · 2013Article
- Comparing and combining capillary electrophoresis electrospray ionization mass spectrometry and nano-liquid chromatography electrospray ionization mass spectrometry for the characterization of post-translationally modified histones.Molecular & cellular proteomics : MCP · 2013Article
- Alterations of histone H1 phosphorylation during bladder carcinogenesis.Journal of proteome research · 2013Article
- High-sensitivity TFA-free LC-MS for profiling histones.Proteomics · 2011Article
- Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow.Aging cell · 2011Article
- Unambiguous determination of isobaric histone modifications by reversed-phase retention time and high-mass accuracy.Analytical biochemistry · 2010Article
- Global histone profiling by LC-FTMS after inhibition and knockdown of deacetylases in human cells.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2009Article
- Enrichment and characterization of histones by two-dimensional hydroxyapatite/reversed-phase liquid chromatography-mass spectrometry.Analytical biochemistry · 2009Article
- Validation of an LC-MS based approach for profiling histones in chronic lymphocytic leukemia.Proteomics · 2009Article
- A robust linear regression based algorithm for automated evaluation of peptide identifications from shotgun proteomics by use of reversed-phase liquid chromatography retention time.BMC bioinformatics · 2008Article
- Mass spectrometry-based strategies for characterization of histones and their post-translational modifications.Expert review of proteomics · 2007Review
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Authors and funding
12 authors.
Funding
Abstract
Here we describe the use of reverse-phase liquid chromatography mass spectrometry (RPLC-MS) to simultaneously characterize variants and post-translationally modified isoforms for each histone. The analysis of intact proteins significantly reduces the time of sample preparation and simplifies data interpretation. LC-MS analysis and peptide mass mapping have previously been applied to identify histone proteins and to characterize their post-translational modifications. However, these studies provided limited characterization of both linker histones and core histones. The current LC-MS analysis allows for the simultaneous observation of all histone PTMs and variants (both replacement and bulk histones) without further enrichment, which will be valuable in comparative studies. Protein identities were verified by the analysis of histone H2A species using RPLC fractionation, AU-PAGE separation and nano-LC-MS/MS.
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