ArticleParasitology research2007
Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri.
Article in Parasitology research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed.
- Cloning of nf-profilin and intercellular interaction with nf-actin in Naegleria fowleri cysts.Scientific reports · 2025Article
- Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1).Parasites, hosts and diseases · 2024Review
- Naegleria fowleri after 50 years: is it a neglected pathogen?Journal of medical microbiology · 2016Article
- Induction of interleukin-8 by Naegleria fowleri lysates requires activation of extracellular signal-regulated kinase in human astroglial cells.Parasitology research · 2012Article
- Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).Parasitology · 2009Article
- Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity.Parasitology research · 2008Article
- Cytopathogenesis of Naegleria fowleri Thai strains for cultured human neuroblastoma cells.Parasitology research · 2008Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A gene encoding a cytosolic heat shock protein 70 from pathogenic Naegleria fowleri (Nf-cHSP70) was identified. The Nf-cHSP70 was 2,062 bp in length with an open reading frame of 1,980 bp encoding 659 amino acid residues. The deduced amino acid sequence of the gene shared high sequence identities with HSP70s from other parasitic organisms and mammals. The characteristic domains, including N-terminal ATPase domain, calmodulin-binding domain, and EE(D)VD motif, found in HSP70s were also well conserved in this gene. The recombinant Nf-cHSP70 protein showed strong antigenicity against the sera from mice experimentally infected with N. fowleri. Immunofluorescence assay showed that Nf-cHSP70 localized in cytosol of the parasite. The results from semi-quantitative RT-PCR and Western blot analyses demonstrated the expression levels of gene transcripts, and its products were significantly increased at high temperature (42 degrees C). The definitive biological roles of Nf-cHSP70 are not clear, but it may protect the parasite under environmental changes especially high temperature.
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Registered trials
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