ArticleMolecular biology of the cell2007
The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.PLoS biology · 2025Article
- The TOG protein Stu2 is regulated by acetylation.PLoS genetics · 2022Article
- Kar9 symmetry breaking alone is insufficient to ensure spindle alignment.Scientific reports · 2021Article
- Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Article
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Mechanism of nuclear movements in a multinucleated cell.Molecular biology of the cell · 2017Article
- Molecular basis of Kar9-Bim1 complex function during mating and spindle positioning.Molecular biology of the cell · 2016Article
- Article
- Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2015Article
- Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.Cytoskeleton (Hoboken, N.J.) · 2015Review
- Dissection of Cdk1-cyclin complexes in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase.Molecular biology of the cell · 2012Article
- The MEN mediates the effects of the spindle assembly checkpoint on Kar9-dependent spindle pole body inheritance in budding yeast.Cell cycle (Georgetown, Tex.) · 2012Article
- Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes.The Journal of cell biology · 2012Article
- Article
- Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes.The Journal of biological chemistry · 2011Article
- Mutation of Ser172 in yeast β tubulin induces defects in microtubule dynamics and cell division.PloS one · 2010Article
- Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.Molecular biology of the cell · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During mitosis in the yeast Saccharomyces cerevisiae, Kar9p directs one spindle pole body (SPB) toward the incipient daughter cell by linking the associated set of cytoplasmic microtubules (cMTs) to the polarized actin network on the bud cortex. The asymmetric localization of Kar9p to one SPB and attached cMTs is dependent on its interactions with microtubule-associated proteins and is regulated by the yeast Cdk1 Cdc28p. Two phosphorylation sites in Kar9p were previously identified. Here, we propose that the two sites are likely to govern Kar9p function through separate mechanisms, each involving a distinct cyclin. In the first mechanism, phosphorylation at serine 496 recruits Kar9p to one SPB. A phosphomimetic mutation at serine 496 bypasses the requirement of BIK1 and CLB5 in generating Kar9p asymmetry. In the second mechanism, Clb4p may target serine 197 of Kar9p for phosphorylation. This modification is required for Kar9p to direct cMTs to the bud. Two-hybrid analysis suggests that this phosphorylation may attenuate the interaction between Kar9p and the XMAP215-homologue Stu2p. We propose that phosphorylation at serine 197 regulates the release of Kar9p from Stu2p at the SPB, either to clear it from the mother-SPB or to allow it to travel to the plus end.
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