ArticleJournal of clinical microbiology2007
Use of dried spots of whole blood, plasma, and mother's milk collected on filter paper for measurement of human immunodeficiency virus type 1 burden.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- The performance of using dried blood spot specimens for HIV-1 viral load testing: A systematic review and meta-analysis.PLoS medicine · 2022Pooled it
- Diagnostic Accuracy of Dried Plasma Spot Specimens for HIV-1 Viral Load Testing: A Systematic Review and Meta-analysis.Journal of acquired immune deficiency syndromes (1999) · 2022Pooled it
- Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions.Frontiers in cellular and infection microbiology · 2025Article
- Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera.Scientific reports · 2023Article
- An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens.Infectious agents and cancer · 2020Article
- Stability of HIV-1 Nucleic Acids in Dried Blood Spot Samples for HIV-1 Drug Resistance Genotyping.PloS one · 2015Article
- An overview of the clinical use of filter paper in the diagnosis of tropical diseases.The American journal of tropical medicine and hygiene · 2014Review
- Article
- Low-cost assays for monitoring HIV infected individuals in resource-limited settings.The Indian journal of medical research · 2011Review
- HIV load testing with small samples of whole blood.Journal of clinical microbiology · 2010Article
- Detection and genotyping of human papillomavirus in self-obtained cervicovaginal samples by using the FTA cartridge: new possibilities for cervical cancer screening.Journal of clinical microbiology · 2009Article
- Monitoring HIV vaccine trial participants for primary infection: studies in the SIV/macaque model.AIDS (London, England) · 2009Article
- Detection of HIV-1 in alternative specimen types using the APTIMA HIV-1 RNA Qualitative Assay.Journal of virological methods · 2009Article
- Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing.Journal of clinical microbiology · 2009Article
- Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing.Journal of clinical microbiology · 2009Article
- Correlation between human immunodeficiency virus type 1 (HIV-1) RNA measurements obtained with dried blood spots and those obtained with plasma by use of Nuclisens EasyQ HIV-1 and Abbott RealTime HIV load tests.Journal of clinical microbiology · 2009Article
- Dried-blood sampling for epstein-barr virus immunoglobulin G (IgG) and IgA serology in nasopharyngeal carcinoma screening.Journal of clinical microbiology · 2008Article
- Feasibility of detecting human immunodeficiency virus type 1 drug resistance in DNA extracted from whole blood or dried blood spots.Journal of clinical microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used for viral load assays, was used for comparison in all our experiments. Utilizing reconstruction experiments, we demonstrate no statistical differences between viral loads determined for plasma and mother's milk spotted on filter paper and those for the same fluids placed directly in lysis buffer. We found that the addition of whole blood directly to lysis buffer was unreliable and could not be considered a feasible option. However, viral load measurements for whole blood spotted onto filter paper correlated with plasma viral load values for both filter spots and lysis buffer (Pearson correlation coefficients, 0.7706 and 0.8155, respectively). In conclusion, dried spots of plasma, whole blood, or mother's milk provide a feasible means for the collection, storage, and shipment of samples for subsequent viral load measurement and monitoring. Virus material spotted and dried on filter paper is a good inexpensive alternative for collecting patient material to monitor the HIV-1 viral load. Measuring the HIV-1 burden from whole blood dried on filter paper provides a suitable alternative for low-technology settings with limited access to refrigeration, as can be found in sub-Saharan Africa.
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