ArticleJournal of clinical microbiology2007
Molecular identification of unusual pathogenic yeast isolates by large ribosomal subunit gene sequencing: 2 years of experience at the United kingdom mycology reference laboratory.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Are the Conventional Commercial Yeast Identification Methods Still Helpful in the Era of New Clinical Microbiology Diagnostics? A Meta-Analysis of Their Accuracy.Journal of clinical microbiology · 2015Pooled it
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- Discrimination of Clinical and Food-DerivedPathogens (Basel, Switzerland) · 2025Article
- Mucor thermorhizoides-A New Species from Post-mining Site in Sudety Mountains (Poland).Current microbiology · 2024Article
- Identification and assessment of antifungal susceptibility ofIranian journal of microbiology · 2024Article
- Changes in fungal taxonomy: mycological rationale and clinical implications.Clinical microbiology reviews · 2023Review
- A Comprehensive Review of Identification Methods for Pathogenic Yeasts: Challenges and Approaches.Advanced biomedical research · 2023Review
- Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.Journal of clinical laboratory analysis · 2022Article
- An Updated Global Species Diversity and Phylogeny in the GenusJournal of fungi (Basel, Switzerland) · 2021Article
- Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom.Antimicrobial agents and chemotherapy · 2019Article
- Identification and antifungal susceptibility profiles ofInfection and drug resistance · 2019Article
- Concordance analysis between different methodologies used for identification of oral isolates ofColombia medica (Cali, Colombia) · 2018Article
- A Fatal Case of Candida auris and Candida tropicalis Candidemia in Neutropenic Patient.Mycopathologia · 2018Article
- Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal CandidiasisIranian biomedical journal · 2017Article
- First Two Cases of Fungal Infections Associated with Multi-drug Resistant Yeast, Fereydounia khargensis.Mycopathologia · 2016Article
- Onychomycosis due to Candida parapsilosis in a Child with Ventricular Septal Defect: An Unusual Predisposition.Case reports in pediatrics · 2016Article
- Development of two molecular approaches for differentiation of clinically relevant yeast species closely related to Candida guilliermondii and Candida famata.Journal of clinical microbiology · 2014Article
- Molecular evidence on the occurrence of co-infection with Pichia guilliermondii and Wuchereria bancrofti in two filarial endemic districts of India.Infectious diseases of poverty · 2014Article
- The usefulness of DNA sequencing after extraction by Whatman FTA filter matrix technology and phenotypic tests for differentiation of Candida albicans and Candida dubliniensis.Mycopathologia · 2014Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid identification of yeast isolates from clinical samples is particularly important given their innately variable antifungal susceptibility profiles. We present here an analysis of the utility of PCR amplification and sequence analysis of the hypervariable D1/D2 region of the 26S rRNA gene for the identification of yeast species submitted to the United Kingdom Mycology Reference Laboratory over a 2-year period. A total of 3,033 clinical isolates were received from 2004 to 2006 encompassing 50 different yeast species. While more than 90% of the isolates, corresponding to the most common Candida species, could be identified by using the AUXACOLOR2 yeast identification kit, 153 isolates (5%), comprised of 47 species, could not be identified by using this system and were subjected to molecular identification via 26S rRNA gene sequencing. These isolates included some common species that exhibited atypical biochemical and phenotypic profiles and also many rarer yeast species that are infrequently encountered in the clinical setting. All 47 species requiring molecular identification were unambiguously identified on the basis of D1/D2 sequences, and the molecular identities correlated well with the observed biochemical profiles of the various organisms. Together, our data underscore the utility of molecular techniques as a reference adjunct to conventional methods of yeast identification. Further, we show that PCR amplification and sequencing of the D1/D2 region reliably identifies more than 45 species of clinically significant yeasts and can also potentially identify new pathogenic yeast species.
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