ArticleThe Journal of molecular diagnostics : JMD2007
Semiautomated multiplexed quantum dot-based in situ hybridization and spectral deconvolution.
Article in The Journal of molecular diagnostics : JMD, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 45 citations in OpenAlex.
- Multicolor multicycle molecular profiling with quantum dots for single-cell analysis.Nature protocols · 2013Article
- Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots.Bioconjugate chemistry · 2011Article
- Designing multifunctional quantum dots for bioimaging, detection, and drug delivery.Chemical Society reviews · 2010Review
- Expression of caveolin-1 in tongue squamous cell carcinoma by quantum dots.European journal of histochemistry : EJH · 2010Article
- Blood and tissue biomarkers in prostate cancer: state of the art.The Urologic clinics of North America · 2010Review
- A prostatic intraepithelial neoplasia-dependent p27 Kip1 checkpoint induces senescence and inhibits cell proliferation and cancer progression.Cancer cell · 2008Article
- Raman nanoparticle probes for antibody-based protein detection in tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Article
- Quantum dots for molecular pathology: their time has arrived.The Journal of molecular diagnostics : JMD · 2007Article
Corrections and comments
- Erratum issuedGossard, Kirk [corrected to Gossage, Kirk]
Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
Abstract
Gene expression profiling has identified several potentially useful gene signatures for predicting outcome or for selecting targeted therapy. However, these signatures have been developed in fresh or frozen tissue, and there is a need to apply them to routinely processed samples. Here, we demonstrate the feasibility of a potentially high-throughput methodology combining automated in situ hybridization with quantum dot-labeled oligonucleotide probes followed by spectral imaging for the detection and subsequent deconvolution of multiple signals. This method is semiautomated and quantitative and can be applied to formalin-fixed, paraffin-embedded tissues. We have combined dual in situ hybridization with immunohistochemistry, enabling simultaneous measurement of gene expression and cell lineage determination. The technique achieves levels of sensitivity and specificity sufficient for the potential application of known expression signatures to biopsy specimens in a semiquantitative way, and the semiautomated nature of the method enables application to high-throughput studies.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.