ArticleNucleic acids research2007
SET3p monomethylates histone H3 on lysine 9 and is required for the silencing of tandemly repeated transgenes in Chlamydomonas.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 39 citations in OpenAlex.
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- DNA cytosine methylation suppresses meiotic recombination at the sex-determining region.Science advances · 2024Article
- Molecular Evolution of Histone Methylation Modification Families in the Plant Kingdom and Their Genome-Wide Analysis in Barley.International journal of molecular sciences · 2023Article
- The epigenetic origin of life history transitions in plants and algae.Plant reproduction · 2021Review
- An Improved Top-Down Mass Spectrometry Characterization ofJournal of the American Society for Mass Spectrometry · 2021Article
- Inhibition of DNA Methylation inFrontiers in genetics · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems.Plant molecular biology · 2016Article
- Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2015Article
- Heat shock factor 1 counteracts epigenetic silencing of nuclear transgenes in Chlamydomonas reinhardtii.Nucleic acids research · 2013Article
- Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii.Plant methods · 2011Article
- Regulation of the Chlamydomonas cell cycle by a stable, chromatin-associated retinoblastoma tumor suppressor complex.The Plant cell · 2010Article
- Micro-algae come of age as a platform for recombinant protein production.Biotechnology letters · 2010Review
- Biofuels from algae: challenges and potential.Biofuels · 2010Article
- Histone modifications associated with drought tolerance in the desert plant Zygophyllum dumosum Boiss.Planta · 2009Article
- Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.Genetics · 2008Article
- Diversification of the core RNA interference machinery in Chlamydomonas reinhardtii and the role of DCL1 in transposon silencing.Genetics · 2008Article
- The MUT9p kinase phosphorylates histone H3 threonine 3 and is necessary for heritable epigenetic silencing in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The highly similar Arabidopsis homologs of trithorax ATX1 and ATX2 encode proteins with divergent biochemical functions.The Plant cell · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
SET domain-containing proteins of the SU(VAR)3-9 class are major regulators of heterochromatin in several eukaryotes, including mammals, insects, plants and fungi. The function of these polypeptides is mediated, at least in part, by their ability to methylate histone H3 on lysine 9 (H3K9). Indeed, mutants defective in SU(VAR)3-9 proteins have implicated di- and/or trimethyl H3K9 in the formation and/or maintenance of heterochromatin across the eukaryotic spectrum. Yet, the biological significance of monomethyl H3K9 has remained unclear because of the lack of mutants exclusively defective in this modification. Interestingly, a SU(VAR)3-9 homolog in the unicellular green alga Chlamydomonas reinhardtii, SET3p, functions in vitro as a specific H3K9 monomethyltransferase. RNAi-mediated suppression of SET3 reactivated the expression of repetitive transgenic arrays and reduced global monomethyl H3K9 levels. Moreover, chromatin immunoprecipitation (ChIP) assays demonstrated that transgene reactivation correlated with the partial loss of monomethyl H3K9 from their chromatin. In contrast, the levels of trimethyl H3K9 or the repression of euchromatic sequences were not affected by SET3 downregulation; whereas dimethyl H3K9 was undetectable in Chlamydomonas. Thus, our observations are consistent with a role for monomethyl H3K9 as an epigenetic mark of repressed chromatin and raise questions as to the functional distinctiveness of different H3K9 methylation states.
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