ArticlePlant molecular biology2007
Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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45 citing papers in PubMed, 1 synthesis or guideline pooled it, 130 citations in OpenAlex.
- A Systematic View Exploring the Role of Chloroplasts in Plant Abiotic Stress Responses.BioMed research international · 2019Pooled it
- Overexpression of cytosolic NADP-malic enzyme 1 from the common ice plant enhances water-deficit and high-light stress tolerance by modulating water-use efficiency and flavonoid biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
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- Genome-Wide Analysis ofPlants (Basel, Switzerland) · 2026Article
- Integrated physiology and multi-omics elucidate photosynthetic adaptation and osmolyte accumulation mechanisms in buffalograss under osmotic stress.BMC plant biology · 2026Article
- Article
- Different Substrates Drive Differential Responses of Rice to Salt Stress.Rice (New York, N.Y.) · 2025Article
- Phytophthora infestans RxLR Effector PiAvr3b Targets NADP-Malic Enzyme to Promote Infection.Molecular plant pathology · 2025Article
- Article
- Candidate genes for alkali tolerance identified by genome-wide association study at the seedling stage in rice (Oryza sativa L.).Scientific reports · 2024Article
- NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice.Rice (New York, N.Y.) · 2024Article
- Transcriptome Analysis for Salt-Responsive Genes in Two Different Alfalfa (Plants (Basel, Switzerland) · 2024Article
- Malic enzyme 1 knockout has no deleterious phenotype and is favored in the male germline under standard laboratory conditions.PloS one · 2024Article
- Individual and combinative effect of NaCl and γ-radiation on NADPH-generating enzymes activity in corn (Heliyon · 2023Article
- Overexpression of chloroplastic Zea mays NADP-malic enzyme (ZmNADP-ME) confers tolerance to salt stress in Arabidopsis thaliana.Photosynthesis research · 2023Article
- Jasmonic Acid Effect onCells · 2023Article
- Transcriptomic Analysis Reveals the Response of the BacteriumApplied and environmental microbiology · 2023Article
- Multi-Omics Approaches Unravel Specific Features of Embryo and Endosperm in Rice Seed Germination.Frontiers in plant science · 2022Article
- ChloroplasticPlants (Basel, Switzerland) · 2021Article
- SSR marker-based genetic diversity analysis and SNP haplotyping of genes associating abiotic and biotic stress tolerance, rice growth and development and yield across 93 rice landraces.Molecular biology reports · 2021Article
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7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plants, and has recently been implicated in plant defense such as in responses to wounding and UV-B radiation. In this study, we isolated a complementary DNA (cDNA) clone by using the differential display method and screening of a root cDNA library of rice (Oryza sativa. L) under carbonate (NaHCO3) stress, and identified it as one of the rice NADP-ME genes (we named it NADP-ME2, GenBank accession no. AB053295). The 5' end of NADP-ME2 was obtained by the 5'-RACE method, and the full-length cDNA had a length of 2217 bp encoding 593 amino acids. Expression of NADP-ME2 mRNA in roots was induced by stress from carbonates (NaHCO3 and Na2CO3, NaCl, and environmental pH changes. NADP-ME2 transcripts increased during 72-h exposures to NaHCO3, NaCl, and PEG stresses. Furthermore, NADP-ME activities in leaves and roots of rice seedlings increased by more than 50% in the presence of carbonates (NaHCO3 and Na2CO3), NaCl, and PEG. These results indicate that rice NADP-ME2 responds to salts and osmotic stresses. Transgenic Arabidopsis plants over-expressing NADP-ME2 were obtained through transformation, screening, Northern analysis and in situ NADP-ME activity assay. Transgenic Arabidopsis plants over-expressing NADP-ME2 grew well in 1/2 x MS medium with 100 mM NaCl or 4% mannitol, whereas growth of wild-type (WT) Arabidopsis seedlings was strongly inhibited. In addition, under 125 mM NaCl stress, the root lengths of transgenic lines were about twice as long as those of the WT. These results suggest that NADP-ME2 has a role in enhancing tolerance of plants to salt and osmotic stress.
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