ArticleProceedings of the National Academy of Sciences of the United States of America2007
RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 90 citations in OpenAlex.
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- Antiviral RISC mainly targets viral mRNA but not genomic RNA of tospovirus.PLoS pathogens · 2021Article
- An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids.International journal of molecular sciences · 2021Review
- Highly efficacious antiviral protection of plants by small interfering RNAs identified in vitro.Nucleic acids research · 2019Article
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- Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein.Genetics and molecular research : GMR · 2016Article
- Isolation and Identification of Post-Transcriptional Gene Silencing-Related Micro-RNAs by Functionalized Silicon Nanowire Field-effect Transistor.Scientific reports · 2015Article
- Roles and programming of Arabidopsis ARGONAUTE proteins during Turnip mosaic virus infection.PLoS pathogens · 2015Article
- AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system.Nucleic acids research · 2013Article
- Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera.Molecular plant pathology · 2013Article
- Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection.The Plant journal : for cell and molecular biology · 2012Article
- Identification of an ARGONAUTE for antiviral RNA silencing in Nicotiana benthamiana.Plant physiology · 2011Article
- ARGONAUTE2 mediates RNA-silencing antiviral defenses against Potato virus X in Arabidopsis.Plant physiology · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19), which is a suppressor of RNAi. Wild-type TBSV or p19-defective mutants initially show a similar infection course in Nicotiana benthamiana, but the absence of an active P19 results in viral RNA degradation followed by recovery from infection. P19 homodimers sequester 21-nt virus-derived duplex siRNAs, and it is thought that this prevents the programming of an antiviral RNA-induced silencing complex to avoid viral RNA degradation. Here we report on chromatographic fractionation (gel filtration, ion exchange, and hydroxyapatite) of extracts from healthy or infected Nicotiana benthamiana plants in combination with in vitro assays for ribonuclease activity and detection of TBSV-derived siRNAs. Only extracts of plants infected with p19 mutants provided a source of sequence-nonspecific but ssRNA-targeted in vitro ribonuclease activity that coeluted with components of a wide molecular weight range. In addition, we isolated a discrete approximately 500-kDa protein complex that contained approximately 21-nt TBSV-derived siRNAs and that exhibited ribonuclease activity that was TBSV sequence-preferential, ssRNA-specific, divalent cation-dependent, and insensitive to a ribonuclease inhibitor. We believe that this study provides biochemical evidence for a virus-host system that infection in the absence of a fully active RNAi suppressor induces ssRNA-specific ribonuclease activity, including that conferred by a RNA-induced silencing complex, which is likely the cause for the recovery of plants from infection.
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