ArticleProceedings of the National Academy of Sciences of the United States of America2007
High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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Who cites it
74 citing papers in PubMed, 128 citations in OpenAlex.
- Microarrays and the Dawn of Omics Data Analysis.Advances in experimental medicine and biology · 2026Review
- Rie1 and Sgn1 form an RNA-binding complex that enforces the meiotic entry cell fate decision.The Journal of cell biology · 2023Article
- Meiosis in budding yeast.Genetics · 2023Article
- The SR-protein Npl3 is an essential component of the meiotic splicing regulatory network in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Saccharomyces cerevisiae RNA lariat debranching enzyme, Dbr1p, is required for completion of reverse transcription by the retrovirus-like element Ty1 and cleaves branched Ty1 RNAs.Molecular genetics and genomics : MGG · 2021Article
- Rules are made to be broken: a "simple" model organism reveals the complexity of gene regulation.Current genetics · 2021Review
- The changing paradigm of intron retention: regulation, ramifications and recipes.Nucleic acids research · 2019Review
- The Paf1 Complex Broadly Impacts the Transcriptome ofGenetics · 2019Article
- Mutagenesis of Snu114 domain IV identifies a developmental role in meiotic splicing.RNA biology · 2019Article
- Excised linear introns regulate growth in yeast.Nature · 2019Article
- Introns in Cryptococcus.Memorias do Instituto Oswaldo Cruz · 2018Review
- The Diversification of Evolutionarily Conserved MAPK Cascades Correlates with the Evolution of Fungal Species and Development of Lifestyles.Genome biology and evolution · 2017Article
- A genome wide dosage suppressor network reveals genomic robustness.Nucleic acids research · 2017Article
- Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events.RNA (New York, N.Y.) · 2016Article
- Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.Nucleic acids research · 2015Article
- The conserved histone deacetylase Rpd3 and its DNA binding subunit Ume6 control dynamic transcript architecture during mitotic growth and meiotic development.Nucleic acids research · 2015Article
- Alternative splicing acting as a bridge in evolution.Stem cell investigation · 2015Article
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
- Article
- Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing.Molecular cell · 2013Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Knowing gene structure is vital to understanding gene function, and accurate genome annotation is essential for understanding cellular function. To this end, we have developed a genome-wide assay for mapping introns in Saccharomyces cerevisiae. Using high-density tiling arrays, we compared wild-type yeast to a mutant deficient for intron degradation. Our method identified 76% of the known introns, confirmed 18 previously predicted introns, and revealed 9 formerly undiscovered introns. Furthermore, we discovered that all 13 meiosis-specific intronic yeast genes undergo regulated splicing, which provides posttranscriptional regulation of the genes involved in yeast cell differentiation. Moreover, we found that approximately 16% of intronic genes in yeast are incompletely spliced during exponential growth in rich medium, which suggests that meiosis is not the only biological process regulated by splicing. Our tiling-array assay provides a snapshot of the spliced transcriptome in yeast. This robust methodology can be used to explore environmentally distinct splicing responses and should be readily adaptable to the study of other organisms, including humans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.