ArticleProceedings of the National Academy of Sciences of the United States of America2007
Imaging synaptosomal calcium concentration microdomains and vesicle fusion by using total internal reflection fluorescent microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- ZIP-ZnT1 complexes mediate a local ZnCellular and molecular life sciences : CMLS · 2026Article
- Calcium Homeostasis, Transporters, and Blockers in Health and Diseases of the Cardiovascular System.International journal of molecular sciences · 2023Review
- Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.Nature biotechnology · 2022Article
- The interplay between mitochondria and store-operated CaJournal of cellular and molecular medicine · 2021Review
- OCIAD1 contributes to neurodegeneration in Alzheimer's disease by inducing mitochondria dysfunction, neuronal vulnerability and synaptic damages.EBioMedicine · 2020Article
- Ammonium chloride alters neuronal excitability and synaptic vesicle release.Scientific reports · 2017Article
- Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Absolute position total internal reflection microscopy with an optical tweezer.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Stress and corticosterone increase the readily releasable pool of glutamate vesicles in synaptic terminals of prefrontal and frontal cortex.Molecular psychiatry · 2014Article
- Properties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Synaptic vesicle exocytosis in hippocampal synaptosomes correlates directly with total mitochondrial volume.Journal of molecular neuroscience : MN · 2013Article
- Subplasmalemmal Ca(2+) measurements in mouse pancreatic beta cells support the existence of an amplifying effect of glucose on insulin secretion.Diabetologia · 2010Article
- Presynaptic calcium signalling in cerebellar mossy fibres.Frontiers in neural circuits · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- Imaging a target of Ca2+ signalling: dense core granule exocytosis viewed by total internal reflection fluorescence microscopy.Methods (San Diego, Calif.) · 2008Article
- The Na-K-ATPase and calcium-signaling microdomains.Physiology (Bethesda, Md.) · 2008Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Transmitter release at chemical synapses is triggered by high calcium concentration microprofiles at the presynaptic cytosol. Such microprofiles, generated by the opening of voltage-dependent calcium channels at the presynaptic plasma membrane, have been defined as calcium concentration microdomains. Using total internal reflection fluorescent microscopy in conjunction with calcium and vesicular release indicator dyes, we have directly visualized the close apposition of calcium concentration microdomains and synaptic release sites at single synaptic terminals from the CNS from rat cerebellar mossy fiber and squid optic lobe. These findings demonstrate the close apposition of calcium entry and release sites and the dynamics of such site locations over time. Kinetic analysis shows that vesicles can be released via two distinct mechanisms: full-fusion and kiss-and-run. Calcium triggers vesicular motion toward the membrane, and the speed of such movement is calcium concentration-dependent. Moreover, the immediately available vesicular pool represents molecularly trapped vesicles that can be located at a larger distance from the plasma membrane than the field illuminated by total internal reflection fluorescent microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.