ArticleRNA (New York, N.Y.)2007
The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 56 citations in OpenAlex.
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- tRNA modifications in cancer: from molecular mechanisms to clinical translation.Biomarker research · 2026Review
- tRNA modifications: greasing the wheels of translation and beyond.RNA biology · 2025Review
- Targeting tRNA methyltransferases: from molecular mechanisms to drug discovery.Science China. Life sciences · 2025Review
- Evolutionary Origins and Functional Diversification of 2'-O-Methyltransferases: Insights from Phylogenetic and Structural Analysis.International journal of molecular sciences · 2025Article
- Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening.BMC plant biology · 2025Article
- Identification of Amino Acids in Trm734 Required for 2'-ACS omega · 2024Article
- Diversity in Biological Function and Mechanism of the tRNA Methyltransferase Trm10.Accounts of chemical research · 2023Article
- Investigations of Single-Subunit tRNA Methyltransferases from Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- Epitranscriptomics as a New Layer of Regulation of Gene Expression in Skeletal Muscle: Known Functions and Future Perspectives.International journal of molecular sciences · 2023Review
- Epitranscriptome: Review of Top 25 Most-Studied RNA Modifications.International journal of molecular sciences · 2022Review
- Developmental roles and molecular mechanisms of Asterix/GTSF1.Wiley interdisciplinary reviews. RNA · 2022Review
- A dual role of human tRNA methyltransferase hTrmt13 in regulating translation and transcription.The EMBO journal · 2022Article
- Ionizing radiation and chemical oxidant exposure impacts on Cryptococcus neoformans transfer RNAs.PloS one · 2022Article
- RNA methylation in chloroplasts or mitochondria in plants.RNA biology · 2021Review
- Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons.Cell reports · 2021Article
- A Census and Categorization Method of Epitranscriptomic Marks.International journal of molecular sciences · 2020Review
- Identification of the enzymes responsible for m2,2G and acp3U formation on cytosolic tRNA from insects and plants.PloS one · 2020Article
- 2'-O-ribose methylation of transfer RNA promotes recovery from oxidative stress in Saccharomyces cerevisiae.PloS one · 2020Article
- A rationale for tRNA modification circuits in the anticodon loop.RNA (New York, N.Y.) · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The methylation of the ribose 2'-OH of RNA occurs widely in nature and in all stable RNAs and occurs at five positions in yeast tRNA. 2'-O-methylation of tRNA at position 4 is interesting because it occurs in the acceptor stem (which is normally undermodified), it is the only 2'-O-methylation that occurs in the middle of a duplex region in tRNA, the modification is conserved in eukaryotes, and the features of the tRNA necessary for substrate recognition are poorly defined. We show here that Saccharomyces cerevisiae ORF YOL125w (TRM13) is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. Biochemical analysis of the S. cerevisiae proteome shows that Trm13 copurifies with 2'-O-methylation activity, using tRNAGlyGCC as a substrate, and extracts made from a trm13-Delta strain have undetectable levels of this activity. Trm13 is necessary for activity in vivo because tRNAs isolated from a trm13-Delta strain lack the corresponding 2'-O-methylated residue for each of the three known tRNAs with this modification. Trm13 is sufficient for 2'-O-methylation at position 4 in vitro since yeast Trm13 protein purified after expression in Escherichia coli has the same activity as that produced in yeast. Trm13 protein binds substrates tRNAHis and tRNAGlyGCC with KD values of 85+/-8 and 100+/-14 nM, respectively, and has a KM for tRNAHis of 10 nM, but binds nonsubstrate tRNAs very poorly (KD>1 microM). Trm13 is conserved in eukaryotes, but there is no sequence similarity between Trm13 and other known methyltransferases.
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