ArticleMolecular and cellular biology2007
Activation status-coupled transient S acylation determines membrane partitioning of a plant Rho-related GTPase.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 115 citations in OpenAlex.
- Functional Characterization of Tomato ShROP7 in Regulating Resistance againstInternational journal of molecular sciences · 2022Article
- ROOT HAIR DEFECTIVE 2 vesicular delivery to the apical plasma membrane domain during Arabidopsis root hair development.Plant physiology · 2022Article
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
- Small GTPases in plant biotic interactions.Small GTPases · 2019Review
- Potato Mop-Top Virus Co-Opts the Stress Sensor HIPP26 for Long-Distance Movement.Plant physiology · 2018Article
- Auxin Signaling.Plant physiology · 2018Review
- The RopGEF2-ROP7/ROP2 Pathway Activated by phyB Suppresses Red Light-Induced Stomatal Opening.Plant physiology · 2017Article
- Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.BioTechniques · 2017Article
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- SHADE AVOIDANCE 4 Is Required for Proper Auxin Distribution in the Hypocotyl.Plant physiology · 2017Article
- Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice.The Plant cell · 2016Article
- GPS-Lipid: a robust tool for the prediction of multiple lipid modification sites.Scientific reports · 2016Article
- Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes.Frontiers in plant science · 2016Article
- Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry.Plant methods · 2016Article
- The connection of cytoskeletal network with plasma membrane and the cell wall.Journal of integrative plant biology · 2015Review
- The small GTPase ROP10 of Medicago truncatula is required for both tip growth of root hairs and nod factor-induced root hair deformation.The Plant cell · 2015Article
- Protein palmitoylation is critical for the polar growth of root hairs in Arabidopsis.BMC plant biology · 2015Article
- Development of an image-based screening system for inhibitors of the plastidial MEP pathway and of protein geranylgeranylation.F1000Research · 2015Article
- Grain setting defect1 (GSD1) function in rice depends on S-acylation and interacts with actin 1 (OsACT1) at its C-terminal.Frontiers in plant science · 2015Article
- The role of lipid post-translational modification in plant developmental processes.Frontiers in plant science · 2014Review
Corrections and comments
- Correction · 2017-09-11Duplication of/in Image · Retract and Replace · Upgrade/Update of Prior Notice(s) · · Retracted article republished with corrections and addition of an author See also: https://pubpeer.com/publications/FA9C72219E23013E12C18FB1CD1F30
- Retraction · 2017-07-28Duplication of/in Image · · Nadav Sorek responsible for figure preparation. See also: https://pubpeer.com/publications/FA9C72219E23013E12C18FB1CD1F30
- Republished as
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ROPs or RACs are plant Rho-related GTPases implicated in the regulation of a multitude of signaling pathways that function at the plasma membrane by virtue of posttranslational lipid modifications. The relationship between ROP activation status and membrane localization has not been established. Here we demonstrate that endogenous ROPs, as well as a transgenic His(6)-green fluorescent protein (GFP)-AtROP6 fusion protein, were partitioned between Triton X-100-soluble and -insoluble membranes. In contrast, an activated His(6)-GFP-Atrop6(CA) mutant protein accumulated exclusively in detergent-resistant membranes. GDP induced accumulation of ROPs in Triton-soluble membranes, whereas GTPgammaS induced accumulation of ROPs in detergent-resistant membranes. Recombinant wild-type and constitutively active AtROP6 isoforms were purified from Arabidopsis plants, and their lipids were cleaved and analyzed by gas chromatography-coupled mass spectrometry. In Triton-soluble membranes, wild-type AtROP6 was only prenylated, primarily by geranylgeranyl. The activated AtROP6 that accumulated in detergent-resistant membranes was modified by prenyl and acyl lipids. The acyl lipids were identified as palmitic and stearic acids. In agreement, activated His(6)-GFP-Atrop6(CA)mS(156) in which cysteine(156) was mutated into serine accumulated in Triton-soluble membranes. These findings show that upon GTP binding and activation, AtROP6 and possibly other ROPs are transiently S acylated, which induces their partitioning into detergent-resistant membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.