ArticleMolecular and cellular biology2007
An inducible enhancer required for Il12b promoter activity in an insulated chromatin environment.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- The difference in immune response and IL-12p35 methylation between newborns and adults.Journal of biomedical science · 2014Trial
- Cohesin-mediated chromatin organization controls the differentiation and function of dendritic cells.Science immunology · 2026Article
- Mutational Analysis of Early, Low-Grade Bowel Polyps Defines a Subgroup with Concurrent, High-Risk Oncogenic Drivers Independent of Polyp Size.Cancer research communications · 2025Article
- Cohesin-mediated chromatin remodeling controls the differentiation and function of conventional dendritic cells.bioRxiv : the preprint server for biology · 2024Article
- IκBζ is a dual-use coactivator of NF-κB and POU transcription factors.Molecular cell · 2024Article
- Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters.Nature communications · 2022Article
- Asthma-associated genetic variants induce IL33 differential expression through an enhancer-blocking regulatory region.Nature communications · 2021Article
- Genetic Basis of Response of Ghanaian Local Chickens to Infection With a Lentogenic Newcastle Disease Virus.Frontiers in genetics · 2020Article
- Article
- Aryl Hydrocarbon Receptor Signaling Cell Intrinsically Inhibits Intestinal Group 2 Innate Lymphoid Cell Function.Immunity · 2018Article
- Inhibition of Inflammatory Gene Transcription by IL-10 Is Associated with Rapid Suppression of Lipopolysaccharide-Induced Enhancer Activation.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Innate immune memory and homeostasis may be conferred through crosstalk between the TLR3 and TLR7 pathways.Science signaling · 2016Article
- The molecular mechanisms of TLR-signaling cooperation in cytokine regulation.Immunology and cell biology · 2016Review
- Epigenetic regulation of the expression of Il12 and Il23 and autoimmune inflammation by the deubiquitinase Trabid.Nature immunology · 2016Article
- Oct1 and OCA-B are selectively required for CD4 memory T cell function.The Journal of experimental medicine · 2015Article
- Transcriptional Activation of Inflammatory Genes: Mechanistic Insight into Selectivity and Diversity.Biomolecules · 2015Review
- Preparing the first responders: building the inflammatory transcriptome from the ground up.Molecular cell · 2014Review
- Oct-1 regulates IL-17 expression by directing interchromosomal associations in conjunction with CTCF in T cells.Molecular cell · 2014Article
- Nucleosomes are stably evicted from enhancers but not promoters upon induction of certain pro-inflammatory genes in mouse macrophages.PloS one · 2014Article
- Oct2 and Obf1 as Facilitators of B:T Cell Collaboration during a Humoral Immune Response.Frontiers in immunology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin-12 (IL-12) and IL-23 are heterodimeric cytokines that serve as critical regulators of T helper cell development. The Il12b gene, which encodes the p40 subunit of both IL-12 and IL-23, is expressed in macrophages and dendritic cells following induction by bacterial products. Although the Il12b promoter, like the promoters of most proinflammatory genes, can support transcriptional induction in typical transfection assays, we show that it is not sufficient for transcription in an insulated chromatin environment. Using a DNase I hypersensitivity assay, two potential distal control regions were identified. One region, DNase I-hypersensitive site 1 (HSS1), located 10 kb upstream of the transcription start site, exhibited hypersensitivity only in stimulated macrophages. In an insulated environment, a 105-bp fragment spanning HSS1 was sufficient for transcription when combined with the Il12b promoter. Although several elements are likely to contribute to activity of the endogenous HSS1 enhancer, including an evolutionarily conserved binding site for C/EBP proteins, the only element required for activity in transient- and stable-transfection assays bound Oct-1 and Oct-2, both of which are expressed constitutively in macrophages. Oct-1 and Oct-2 were recruited to the enhancer upon macrophage stimulation, and the Oct site appeared important for nucleosome remodeling at HSS1. These results suggest that the HSS1 enhancer and Oct proteins play central roles in Il12b induction upon macrophage activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.