ArticleMolecular and cellular biology2007
Iron-dependent degradation of apo-IRP1 by the ubiquitin-proteasome pathway.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
- An exploratory clinical trial of bortezomib in patients with lower risk myelodysplastic syndromes.American journal of hematology · 2017Trial
- Ferroportin at the Crossroads of Iron Biology: Disease, Regulation and Modulation.Biomolecules · 2026Review
- Identification of Pinostilbene as a natural STING agonist that triggers FTH1 degradation via K48-ubiquitination to induce ferroptosis in non-small cell lung cancer.Redox biology · 2026Article
- Mitochondrial Aconitase ACO2 Links Iron Homeostasis with Tumorigenicity in Non-Small Cell Lung Cancer.Molecular cancer research : MCR · 2023Article
- Expanding the phenotypic and molecular spectrum of NFS1-related disorders that cause functional deficiencies in mitochondrial and cytosolic iron-sulfur cluster containing enzymes.Human mutation · 2022Article
- Benefits of Iron Chelators in the Treatment of Parkinson's Disease.Neurochemical research · 2021Article
- "Metal elements and pesticides as risk factors for Parkinson's disease - A review".Toxicology reports · 2021Review
- The S-nitrosylation of parkin attenuated the ubiquitination of divalent metal transporter 1 in MPPScientific reports · 2020Article
- Iron-responsive-like elements and neurodegenerative ferroptosis.Learning & memory (Cold Spring Harbor, N.Y.) · 2020Review
- Iron Regulation: Macrophages in Control.Pharmaceuticals (Basel, Switzerland) · 2018Review
- RNA-protein interaction detection in living cells.Nature methods · 2018Article
- A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.The Journal of biological chemistry · 2017Article
- Brain Iron Metabolism Dysfunction in Parkinson's Disease.Molecular neurobiology · 2017Review
- Macrophagic control of the response to uropathogenicImmunity, inflammation and disease · 2016Article
- Parkinson's Disease: The Mitochondria-Iron Link.Parkinson's disease · 2016Review
- Bacillithiol has a role in Fe-S cluster biogenesis in Staphylococcus aureus.Molecular microbiology · 2015Article
- The IRP/IRE system in vivo: insights from mouse models.Frontiers in pharmacology · 2014Review
- Dysregulation of cellular iron metabolism in Friedreich ataxia: from primary iron-sulfur cluster deficit to mitochondrial iron accumulation.Frontiers in pharmacology · 2014Review
- Protective effect of mitochondrial ferritin on cytosolic iron dysregulation induced by doxorubicin in HeLa cells.Molecular biology reports · 2013Article
- Iron increases the susceptibility of multiple myeloma cells to bortezomib.Haematologica · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron regulatory protein 1 (IRP1) controls the translation or stability of several mRNAs by binding to "iron-responsive elements" within their untranslated regions. In iron-replete cells, IRP1 assembles a cubane iron-sulfur cluster (ISC) that inhibits RNA-binding activity and converts the protein to cytosolic aconitase. We show that the constitutive IRP1(C437S) mutant, which fails to form an ISC, is destabilized by iron. Thus, exposure of H1299 cells to ferric ammonium citrate reduced the half-life of transfected IRP1(C437S) from approximately 24 h to approximately 10 h. The iron-dependent degradation of IRP1(C437S) involved ubiquitination, required ongoing transcription and translation, and could be efficiently blocked by the proteasomal inhibitors MG132 and lactacystin. Similar results were obtained with overexpressed wild-type IRP1, which predominated in the apo-form even in iron-loaded H1299 cells, possibly due to saturation of the ISC assembly machinery. Importantly, inhibition of ISC biogenesis in HeLa cells by small interfering RNA knockdown of the cysteine desulfurase Nfs1 sensitized endogenous IRP1 for iron-dependent degradation. Collectively, these data uncover a mechanism for the regulation of IRP1 abundance as a means to control its RNA-binding activity, when the ISC assembly pathway is impaired.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.