ArticleExperimental cell research2007
Erk1/2 MAPK and caldesmon differentially regulate podosome dynamics in A7r5 vascular smooth muscle cells.
Article in Experimental cell research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.Nature communications · 2026Article
- Beyond Blood Pressure: Emerging Pathways and Precision Approaches in Hypertension-Induced Kidney Damage.International journal of molecular sciences · 2025Review
- Caldesmon ablation in mice causes umbilical herniation and alters contractility of fetal urinary bladder smooth muscle.The Journal of general physiology · 2021Article
- Dark-Side of Exosomes.Advances in experimental medicine and biology · 2021Article
- Caldesmon: Biochemical and Clinical Implications in Cancer.Frontiers in cell and developmental biology · 2021Review
- L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts.Journal of biomedical science · 2019Article
- Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta.Biomolecules & therapeutics · 2018Article
- Circulating Exosomes Isolated from Septic Mice Induce Cardiovascular Hyperpermeability Through Promoting Podosome Cluster Formation.Shock (Augusta, Ga.) · 2018Article
- Hypothermia Inhibits Endothelium-Independent Vascular Contractility via Rho-kinase Inhibition.Biomolecules & therapeutics · 2018Article
- Invadosomes are coming: new insights into function and disease relevance.The FEBS journal · 2018Review
- Matrix Metalloproteinases -14, -9 and -2 are Localized to the Podosome and Involved in Podosome Development in the A7r5 Smooth Muscle Cell.Journal of cardiobiology · 2017Article
- Endothelium-Independent Effect of Fisetin on the Agonist-Induced Regulation of Vascular Contractility.Biomolecules & therapeutics · 2016Article
- Cardamonin inhibits agonist-induced vascular contractility via Rho-kinase and MEK inhibition.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2016Article
- Article
- The inhibitory effect of shikonin on the agonist-induced regulation of vascular contractility.Biomolecules & therapeutics · 2015Article
- The Inhibitory Effect of Apigenin on the Agonist-Induced Regulation of Vascular Contractility via Calcium Desensitization-Related Pathways.Biomolecules & therapeutics · 2014Article
- ADAMTS-4 and biglycan are expressed at high levels and co-localize to podosomes during endothelial cell tubulogenesis in vitro.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Nicotinic acetylcholine receptor mediates nicotine-induced actin cytoskeletal remodeling and extracellular matrix degradation by vascular smooth muscle cells.Vascular pharmacology · 2013Article
- Stimulus-specific activation and actin dependency of distinct, spatially separated ERK1/2 fractions in A7r5 smooth muscle cells.PloS one · 2012Article
- Caldesmon regulates actin dynamics to influence cranial neural crest migration in Xenopus.Molecular biology of the cell · 2011Article
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Authors and funding
5 authors.
Funding
Abstract
We tested the hypothesis that the MEK/Erk/caldesmon phosphorylation cascade regulates PKC-mediated podosome dynamics in A7r5 cells. We observed the phosphorylation of MEK, Erk and caldesmon, and their translocation to the podosomes upon phorbol dibutyrate (PDBu) stimulation, together with the nuclear translocation of phospho-MEK and phospho-Erk. After MEK inhibition by U0126, Erk translocated to the interconnected actin-rich columns but failed to translocate to the nucleus, suggesting that podosomes served as a site for Erk phosphorylation. The interconnected actin-rich columns in U0126-treated, PDBu-stimulated cells contained alpha-actinin, caldesmon, vinculin, and metalloproteinase-2. Caldesmon and vinculin became integrated with F-actin at the columns, in contrast to their typical location at the ring of podosomes. Live-imaging experiments suggested the growth of these columns from podosomes that were slow to disassemble. The observed modulation of podosome size and life time in A7r5 cells overexpressing wild-type and phosphorylation-deficient caldesmon-GFP mutants in comparison to untransfected cells suggests that caldesmon and caldesmon phosphorylation modulate podosome dynamics in A7r5 cells. These results suggest that Erk1/2 and caldesmon differentially modulate PKC-mediated formation and/or dynamics of podosomes in A7r5 vascular smooth muscle cells.
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