ArticlePlant molecular biology2007
Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.).
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 143 citations in OpenAlex.
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- The molecular evolutionary basis of species formation revisited.Trends in genetics : TIG · 2025Review
- The cytonuclear interactions during grapevine domestication.Journal of integrative plant biology · 2025Article
- Mitochondrial candidate gene,Molecular breeding : new strategies in plant improvement · 2025Article
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- Comparative Analysis of the Mitochondrial Genome of Eggplant (International journal of molecular sciences · 2024Article
- Insights into cellular crosstalk regulating cytoplasmic male sterility and fertility restoration.Molecular biology reports · 2024Review
- Fine mapping and candidate gene analysis of a novel fertility restorer gene for C-type cytoplasmic male sterility in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Genetic Mechanisms for Hybrid Breeding in Vegetable Crops.Plants (Basel, Switzerland) · 2023Review
- Development of male sterile lines of CMS chilies (Breeding science · 2023Article
- Fine mapping of Rf2, a minor Restorer-of-fertility (Rf) gene for cytoplasmic male sterility in chili pepper G164 (Capsicum annuum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Orf165 is associated with cytoplasmic male sterility in pepper.Genetics and molecular biology · 2021Article
- Genome-wide analysis of mRNA and lncRNA expression and mitochondrial genome sequencing provide insights into the mechanisms underlying a novel cytoplasmic male sterility system, BVRC-CMS96, in Brassicarapa.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Fine mapping of the male fertility restoration gene CaRf032 in Capsicum annuum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- A predicted NEDD8 conjugating enzyme gene identified as aHorticulture research · 2020Article
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- Organelle genome composition and candidate gene identification for Nsa cytoplasmic male sterility in Brassica napus.BMC genomics · 2019Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) in plants is known to be associated with novel open reading frames (ORFs) that result from recombination events in the mitochondrial genome. In this study Southern and Northern blot analyses using several mitochondrial DNA probes were conducted to detect the presence of differing band patterns between male fertile and CMS lines of chili pepper (Capsicum annuum L.). In the CMS pepper, a novel ORF, termed orf456, was found at the 3'-end of the coxll gene. Western blot analysis revealed the expression of an approximately 17-kDa product in the CMS line, and the intensity of expression of this protein was severely reduced in the restorer pepper line. To investigate the functional role of the ORF456 protein in plant mitochondria, we carried out two independent experiments to transform Arabidopsis with a mitochondrion-targeted orf456 gene construct by Agrobacterium-mediated transformation. About 45 % of the T1 transgenic population showed the male-sterile phenotype and no seed set. Pollen grains from semi-sterile T1 plants were observed to have defects on the exine layer and vacuolated pollen phenotypes. It is concluded that this newly discovered orf456 may represent a strong candidate gene--from among the many CMS-associated mitochondrial genes--for determining the male-sterile phenotype of CMS in chili pepper.
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