ArticleTransgenic research2007
Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- TheGenes · 2026Article
- A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Targeted insertion of regulatory elements enables translational enhancement in rice.Frontiers in plant science · 2023Article
- Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.BMC plant biology · 2018Article
- A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don.Scientific reports · 2016Article
- Characterization of a maize Wip1 promoter in transgenic plants.International journal of molecular sciences · 2013Article
- Heterologous expression of the wheat aquaporin gene TaTIP2;2 compromises the abiotic stress tolerance of Arabidopsis thaliana.PloS one · 2013Article
- Endosperm-specific expression of human acid beta-glucosidase in a waxy rice.Rice (New York, N.Y.) · 2012Article
- A 28 nt long synthetic 5'UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants.BMC biotechnology · 2012Article
- Tackling heterogeneity: a leaf disc-based assay for the high-throughput screening of transient gene expression in tobacco.PloS one · 2012Article
- The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational enhancer in plants.Transgenic research · 2010Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve expression levels of recombinant proteins in plants, a new leader sequence was designed. Several elements known to enhance gene translation and/or transcription were considered, including the CaMV 35S Inr site, a CT-rich motif often shared by highly expressed plant genes and a poly(CAA) region widespread in tobamovirus and plant leaders. The effect of the synthetic leader on gusA expression was evaluated in genetically modified tobacco plants by measuring the beta-glucuronidase activity and the mRNA level. When compared to the gusA leader of pBI121, the new sequence determined a 8.6-fold and a 12.5-fold increase of enzyme concentration taking into account the whole plant population or the above-average expressors, respectively. Since most pCAMBIA vectors harbour a very short 5'-UTR, identical to a fragment of the pBI121 leader, leader replacement with the sequence herein described is strongly suggested.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.