ArticleJournal of bacteriology2007
Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Molecular Cloning, Expression and Characterization of Oenococcus oeni Priming Glycosyltransferases.Molecular biotechnology · 2017Article
- Emerging facets of prokaryotic glycosylation.FEMS microbiology reviews · 2017Review
- Outer membrane vesicles of Tannerella forsythia: biogenesis, composition, and virulence.Molecular oral microbiology · 2015Article
- Topological analysis of the Escherichia coli WcaJ protein reveals a new conserved configuration for the polyisoprenyl-phosphate hexose-1-phosphate transferase family.Scientific reports · 2015Article
- Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli.Structure (London, England : 1993) · 2013Article
- "Cross-glycosylation" of proteins in Bacteroidales species.Glycobiology · 2013Article
- Bacterial cell-envelope glycoconjugates.Advances in carbohydrate chemistry and biochemistry · 2013Review
- The novel virulence-related gene nlxA in the lipopolysaccharide cluster of Xanthomonas citri ssp. citri is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance.Molecular plant pathology · 2012Article
- Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase.Protein science : a publication of the Protein Society · 2012Article
- Functional characterization of UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferases of Escherichia coli and Caulobacter crescentus.Journal of bacteriology · 2012Article
- Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia.The Journal of biological chemistry · 2011Article
- The s-layer glycome-adding to the sugar coat of bacteria.International journal of microbiology · 2011Article
- ABC transporters involved in export of cell surface glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2010Review
- The complete genome of Zunongwangia profunda SM-A87 reveals its adaptation to the deep-sea environment and ecological role in sedimentary organic nitrogen degradation.BMC genomics · 2010Article
- Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus.Journal of molecular biology · 2010Article
- Construction of a gene knockout system for application in Paenibacillus alvei CCM 2051T, exemplified by the S-layer glycan biosynthesis initiation enzyme WsfP.Applied and environmental microbiology · 2009Article
- Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps.Journal of bacteriology · 2008Article
- Recombinant glycans on an S-layer self-assembly protein: a new dimension for nanopatterned biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2008Article
- S-layer nanoglycobiology of bacteria.Carbohydrate research · 2008Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
The glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-->2)-alpha-l-Rhap-(1-->3)-beta-l-Rhap-(1-->2)-alpha-l-Rhap-(1-->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-l-rhamnose residues, and a beta-d-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium.
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