ArticleJournal of bacteriology2007
Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Some Like it Hot: Efficiency of the Type III Secretion System has Multiple Thermosensitive Behaviours in the Pseudomonas syringae Complex.Molecular plant pathology · 2025Article
- Phaseolotoxin: Environmental Conditions and Regulatory Mechanisms Involved in Its Synthesis.Microorganisms · 2024Review
- Comparative genomics reveals insights into the potential of Lysinibacillus irui as a plant growth promoter.Applied microbiology and biotechnology · 2024Article
- Natural Products That Contain Higher Homologated Amino Acids.Chembiochem : a European journal of chemical biology · 2024Review
- OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production inMicroorganisms · 2022Article
- The Pbo Cluster fromToxins · 2021Article
- Two Homologues of the Global Regulator Csr/Rsm Redundantly Control Phaseolotoxin Biosynthesis and Virulence in the Plant PathogenMicroorganisms · 2020Article
- Recent developments in self-resistance gene directed natural product discovery.Natural product reports · 2020Review
- Biosynthetic reconstitution of deoxysugar phosphoramidate metalloprotease inhibitors using an N-P-bond-forming kinase.Chemical science · 2019Article
- Plant-Pathogen Warfare under Changing Climate Conditions.Current biology : CB · 2018Review
- Four genes essential for recombination define GInts, a new type of mobile genomic island widespread in bacteria.Scientific reports · 2017Article
- Article
- Characterization of AmtA, an amidinotransferase involved in the biosynthesis of phaseolotoxins.FEBS open bio · 2016Article
- Ornithine Transcarbamylase ArgK Plays a Dual role for the Self-defense of Phaseolotoxin Producing Pseudomonas syringae pv. phaseolicola.Scientific reports · 2015Article
- Enzymatic strategies and biocatalysts for amide bond formation: tricks of the trade outside of the ribosome.Molecular bioSystems · 2015Review
- Article
- L-amino acid ligase from Pseudomonas syringae producing tabtoxin can be used for enzymatic synthesis of various functional peptides.Applied and environmental microbiology · 2013Article
- Transcriptional profile of P. syringae pv. phaseolicola NPS3121 at low temperature: physiology of phytopathogenic bacteria.BMC microbiology · 2013Article
- Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production.BMC microbiology · 2012Article
- The mbo operon is specific and essential for biosynthesis of mangotoxin in Pseudomonas syringae.PloS one · 2012Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas syringae pv. phaseolicola is the causal agent of halo blight disease of beans (Phaseolus vulgaris L.), which is characterized by water-soaked lesions surrounded by a chlorotic halo resulting from the action of a non-host-specific toxin known as phaseolotoxin. This phytotoxin inhibits the enzyme ornithine carbamoyltransferase involved in arginine biosynthesis. Different evidence suggested that genes involved in phaseolotoxin production were clustered. Two genes had been previously identified in our laboratory within this cluster: argK, which is involved in the immunity of the bacterium to its own toxin, and amtA, which is involved in the synthesis of homoarginine. We sequenced the region around argK and amtA in P. syringae pv. phaseolicola NPS3121 to determine the limits of the putative phaseolotoxin gene cluster and to determine the transcriptional pattern of the genes comprising it. We report that the phaseolotoxin cluster (Pht cluster) is composed of 23 genes and is flanked by insertion sequences and transposases. The mutation of 14 of the genes within the cluster lead to a Tox(-) phenotype for 11 of them, while three mutants exhibited low levels of toxin production. The analysis of fusions of selected DNA fragments to uidA, Northern probing, and reverse transcription-PCR indicate the presence of five transcriptional units, two monocistronic and three polycistronic; one is internal to a larger operon. The site for transcription initiation has been determined for each promoter, and the putative promoter regions were identified. Preliminary results also indicate that the gene product of phtL is involved in the regulation of the synthesis of phaseolotoxin.
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