ArticleStem cells (Dayton, Ohio)2007
Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations.
Article in Stem cells (Dayton, Ohio), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons.Stem cell research · 2019Article
- Potential and Challenges of Induced Pluripotent Stem Cells in Liver Diseases Treatment.Journal of clinical medicine · 2014Review
- Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells.Stem cells translational medicine · 2014Article
- Directed midbrain and spinal cord neurogenesis from pluripotent stem cells to model development and disease in a dish.Frontiers in neuroscience · 2014Review
- Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors.BMC biology · 2013Article
- Pluripotent stem cells for Parkinson's disease: progress and challenges.Stem cell research & therapy · 2013Review
- Stem cell grafting improves both motor and cognitive impairments in a genetic model of Parkinson's disease, the aphakia (ak) mouse.Cell transplantation · 2013Article
- Adult human olfactory epithelial-derived progenitors: a potential autologous source for cell-based treatment for Parkinson's disease.Stem cells translational medicine · 2012Article
- GABAergic neurons from mouse embryonic stem cells possess functional properties of striatal neurons in vitro, and develop into striatal neurons in vivo in a mouse model of Huntington's disease.Stem cell reviews and reports · 2012Article
- Using stem cells and iPS cells to discover new treatments for Parkinson's disease.Parkinsonism & related disorders · 2012Review
- Highly pure and expandable PSA-NCAM-positive neural precursors from human ESC and iPSC-derived neural rosettes.PloS one · 2012Article
- Cellular programming and reprogramming: sculpting cell fate for the production of dopamine neurons for cell therapy.Stem cells international · 2012Article
- Functional integration of dopaminergic neurons directly converted from mouse fibroblasts.Cell stem cell · 2011Article
- Induced pluripotent stem cells (iPSCs) and neurological disease modeling: progress and promises.Human molecular genetics · 2011Review
- Microfluidics for cell separation.Medical & biological engineering & computing · 2010Review
- Stem cell-based neuroprotective and neurorestorative strategies.International journal of molecular sciences · 2010Review
- High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Upping the ante: recent advances in direct reprogramming.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Human embryonic stem cells which express hrGFP in the undifferentiated state and during dopaminergic differentiation.Restorative neurology and neuroscience · 2009Article
- Embryonic stem cell-derived Pitx3-enhanced green fluorescent protein midbrain dopamine neurons survive enrichment by fluorescence-activated cell sorting and function in an animal model of Parkinson's disease.Stem cells (Dayton, Ohio) · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Transplantation of mouse embryonic stem (mES) cells can restore function in Parkinson disease models, but can generate teratomas. Purification of dopamine neurons derived from embryonic stem cells by fluorescence-activated cell sorting (FACS) could provide a functional cell population for transplantation while eliminating the risk of teratoma formation. Here we used the tyrosine hydroxylase (TH) promoter to drive enhanced green fluorescent protein (eGFP) expression in mES cells. First, we evaluated 2.5-kilobase (kb) and 9-kb TH promoter fragments and showed that clones generated using the 9-kb fragment produced significantly more eGFP+/TH+ neurons. We selected the 9-kb TH clone with the highest eGFP/TH overlap for further differentiation, FACS, and transplantation experiments. Grafts contained large numbers of eGFP+ dopamine neurons of an appropriate phenotype. However, there were also numerous eGFP+ cells that did not express TH and did not have a neuronal morphology. In addition, we found cells in the grafts representing all three germ layers. Based on these findings, we examined the expression of stem cell markers in our eGFP+ population. We found that a majority of eGFP+ cells were stage-specific embryonic antigen-positive (SSEA-1+) and that the genetically engineered clones contained more SSEA-1+ cells after differentiation than the original D3 mES cells. By negative selection of SSEA-1, we could isolate a neuronal eGFP+ population of high purity. These results illustrate the complexity of using genetic selection to purify mES cell-derived dopamine neurons and provide a comprehensive analysis of cell selection strategies based on tyrosine hydroxylase expression. Disclosure of potential conflicts of interest is found at the end of this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.