ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
The RNA-binding protein HuD binds acetylcholinesterase mRNA in neurons and regulates its expression after axotomy.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- ELAVL Proteins, miRNA Fate, and Extracellular RNA Communication in Brain Aging and Neurodegeneration.Cells · 2026Review
- The RNA-Binding Protein ELAVL4/HuD Promotes Stress-Associated Tumor Survival in Neuroendocrine Cancers.International journal of molecular sciences · 2026Review
- Protein Predictors of Worsening Cerebral Edema in Traumatic Brain Injury in Critically Ill Patients.bioRxiv : the preprint server for biology · 2026Article
- Emerging Roles for the RNA-Binding Protein HuD (ELAVL4) in Nervous System Diseases.International journal of molecular sciences · 2022Review
- RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons.Progress in neurobiology · 2022Article
- Roles of Embryonic Lethal Abnormal Vision-Like RNA Binding Proteins in Cancer and Beyond.Frontiers in cell and developmental biology · 2022Review
- Review
- Urokinase-type plasminogen activator (uPA) regulates the expression and function of growth-associated protein 43 (GAP-43) in the synapse.The Journal of biological chemistry · 2020Article
- Motor neuron biology and disease: A current perspective on infantile-onset spinal muscular atrophy.Future neurology · 2018Article
- HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA.Molecular cell · 2018Article
- A Shift from a Pivotal to Supporting Role for the Growth-Associated Protein (GAP-43) in the Coordination of Axonal Structural and Functional Plasticity.Frontiers in cellular neuroscience · 2017Review
- Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction.RNA (New York, N.Y.) · 2013Review
- Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1.The Journal of cell biology · 2013Article
- Characterization of multiple exon 1 variants in mammalian HuD mRNA and neuron-specific transcriptional control via neurogenin 2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Trans-acting factors governing acetylcholinesterase mRNA metabolism in neurons.Frontiers in molecular neuroscience · 2012Article
- RNA-binding protein FXR2 regulates adult hippocampal neurogenesis by reducing Noggin expression.Neuron · 2011Article
- HuD regulates the cpg15 expression via the 3'-UTR and AU-rich element.Neurochemical research · 2011Article
- Increased expression of axogenesis-related genes and mossy fibre length in dentate granule cells from adult HuD overexpressor mice.ASN neuro · 2011Article
- Novel recognition motifs and biological functions of the RNA-binding protein HuD revealed by genome-wide identification of its targets.Nucleic acids research · 2010Article
- Regulation of heme oxygenase-1 mRNA deadenylation and turnover in NIH3T3 cells by nitrosative or alkylation stress.BMC molecular biology · 2007Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
After axotomy, expression of acetylcholinesterase (AChE) is greatly reduced in the superior cervical ganglion (SCG); however, the molecular events involved in this response remain unknown. Here, we first examined AChE mRNA levels in the brain of transgenic mice that overexpress human HuD. Both in situ hybridization and reverse transcription-PCR demonstrated that AChE transcript levels were increased by more than twofold in the hippocampus of HuD transgenic mice. Additionally, direct interaction between the HuD transgene product and AChE mRNA was observed. Next, we examined the role of HuD in regulating AChE expression in intact and axotomized rat SCG neurons. After axotomy of the adult rat SCG neurons, AChE transcript levels decreased by 50 and 85% by the first and fourth day, respectively. In vitro mRNA decay assays indicated that the decrease in AChE mRNA levels resulted from changes in the stability of presynthesized transcripts. A combination of approaches performed using the region that directly encompasses an adenylate and uridylate (AU)-rich element within the AChE 3'-untranslated region demonstrated a decrease in RNA-protein complexes in response to axotomy of the SCG and, specifically, a decrease in HuD binding. After axotomy, HuD transcript and protein levels also decreased. Using a herpes simplex virus construct containing the human HuD sequence to infect SCG neurons in vivo, we found that AChE and GAP-43 mRNA levels were maintained in the SCG after axotomy. Together, the results of this study demonstrate that AChE expression in neurons of the rat SCG is regulated via post-transcriptional mechanisms that involve the AU-rich element and HuD.
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