ArticleDevelopmental biology2007
Tough beginnings: alterations in the transcriptome of cloned embryos during the first two cell cycles.
Article in Developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 74 citations in OpenAlex.
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming.Scientific reports · 2020Article
- A Comparative Analysis of Single-Cell Transcriptome Identifies Reprogramming Driver Factors for Efficiency Improvement.Molecular therapy. Nucleic acids · 2020Article
- Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing.BMC genomics · 2018Article
- RNAi-mediated knockdown ofOncotarget · 2017Article
- KDM4B-mediated reduction of H3K9me3 and H3K36me3 levels improves somatic cell reprogramming into pluripotency.Scientific reports · 2017Article
- Sperm is epigenetically programmed to regulate gene transcription in embryos.Genome research · 2016Article
- Article
- RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming.PloS one · 2016Article
- Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer.Scientific reports · 2015Article
- Article
- Article
- Reshaping the transcriptional frontier: epigenetics and somatic cell nuclear transfer.Molecular reproduction and development · 2014Article
- Contribution of CBX4 to cumulus oophorus cell phenotype in mice and attendant effects in cumulus cell cloned embryos.Physiological genomics · 2014Article
- Interspecies somatic cell nuclear transfer: advancements and problems.Cellular reprogramming · 2013Review
- The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal–spatial-specific reprogramming.Reproduction (Cambridge, England) · 2013Article
- Systems genetics implicates cytoskeletal genes in oocyte control of cloned embryo quality.Genetics · 2013Article
- Nuclear reprogramming of sperm and somatic nuclei in eggs and oocytes.Reproductive medicine and biology · 2013Article
- Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.Biology of reproduction · 2013Article
- More with less Xist.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cloned embryos produced by somatic cell nuclear transfer (SCNT) display a plethora of phenotypic characteristics that make them different from fertilized embryos, indicating defects in the process of nuclear reprogramming by the recipient ooplasm. To elucidate the extent and timing of nuclear reprogramming, we used microarrays to analyze the transcriptome of mouse SCNT embryos during the first two cell cycles. We identified a large number of genes mis-expressed in SCNT embryos. We found that genes involved in transcription and regulation of transcription are prominent among affected genes, and thus may be particularly difficult to reprogram, and these likely cause a ripple effect that alters the transcriptome of many other functions, including oxidative phosphorylation, transport across membrane, and mRNA transport and processing. Interestingly, we also uncovered widespread alterations in the maternal (i.e., non-transcribed) mRNA population of SCNT embryos. We conclude that gene expression in early SCNT embryos is grossly abnormal, and that this is at least in part the result of incomplete reprogramming of transcription factor genes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.