ArticleCancer science2007
Downregulation of RECK by promoter methylation correlates with lymph node metastasis in non-small cell lung cancer.
Article in Cancer science, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- The Roles of the Membrane-Anchored Glycoprotein RECK in Animal Development, Tumor Suppression, and Beyond.Life (Basel, Switzerland) · 2026Review
- Identification and validation of DNA methylation markers to predict axillary lymph node metastasis of breast cancer.PloS one · 2022Article
- Review
- MicroRNA-221 promotes papillary thyroid carcinoma cell migration and invasion via targeting RECK and regulating epithelial-mesenchymal transition.OncoTargets and therapy · 2019Article
- Identification ofOncotarget · 2018Article
- Promoter hypermethylation of the RECK gene is associated with its low expression and poor survival of esophageal squamous cell carcinoma.Oncology letters · 2017Article
- Pattern of RECK CpG methylation as a potential marker for predicting breast cancer prognosis and drug-sensitivity.Oncotarget · 2016Article
- Epigenetic silencing of Kruppel like factor-3 increases expression of pro-metastatic miR-182.Cancer letters · 2015Article
- Ras induces experimental lung metastasis through up-regulation of RbAp46 to suppress RECK promoter activity.BMC cancer · 2015Article
- Effects of 5-aza-2'deoxycytidine on RECK gene expression and tumor invasion in salivary adenoid cystic carcinoma.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region.Cancer gene therapy · 2014Article
- Article
- Valproic acid affected the survival and invasiveness of human glioma cells through diverse mechanisms.Journal of neuro-oncology · 2012Article
- The metalloproteinase inhibitor Reck is essential for zebrafish DRG development.Development (Cambridge, England) · 2012Article
- Effect of RECK gene polymorphisms on hepatocellular carcinoma susceptibility and clinicopathologic features.PloS one · 2012Article
- The silencing of RECK gene is associated with promoter hypermethylation and poor survival in hepatocellular carcinoma.International journal of biological sciences · 2012Article
- Integrated mutation, copy number and expression profiling in resectable non-small cell lung cancer.BMC cancer · 2011Article
- Reversion-inducing cysteine-rich protein with Kazal motifs interferes with epidermal growth factor receptor signaling.Oncogene · 2011Article
- The chemopreventive properties and therapeutic modulation of green tea polyphenols in oral squamous cell carcinoma.ISRN oncology · 2011Article
- [Advances of DNA methylation in lung cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we addressed the molecular mechanism of the downregulation of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK), a critical tumor suppressor that can potently inhibit angiogenesis and metastasis, in non-small cell lung cancer and its clinical significance. The methylation status of the RECK gene promoter was studied by methylation-specific polymerase chain reaction. RECK mRNA and protein levels were investigated by reverse transcription-polymerase chain reaction and western blot analysis. Downregulation of RECK was observed in 60% of the 55 tumors analyzed. Using methylation-specific polymerase chain reaction analysis methylation of the RECK promoter was detected in 63.6% (35/55) of the tumor tissues. A strong correlation between downregulation and promoter methylation was found in these tumors (P = 0.000005). More importantly, downregulation of RECK significantly correlated with lymph node metastasis (P = 0.038). Mutation of codon 12 of the K-ras gene was detected in 25.5% (14/55) of lung tumor tissues. Statistical analysis indicated that K-ras mutation was linked with RECK promoter methylation (P = 0.047) and downregulation (P = 0.023). Promoter methylation was also detected in human lung cancer cell lines, and the DNA methyltransferase inhibitor 5'-azacytidine reversed the expression of RECK and reduced the invasive ability of these cell lines. Collectively, our results suggest that downregulation of the metastasis suppressor RECK is caused by promoter methylation in non-small cell lung cancer and is associated with K-ras mutation and lymph node metastasis.
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Registered trials
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