ArticleBiophysical journal1991
Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 238 citations in OpenAlex.
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- Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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- Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging.Materials today. Bio · 2024Article
- Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning.PNAS nexus · 2023Article
- Review of Mn-Doped Semiconductor Nanocrystals for Time-Resolved Luminescence Biosensing/Imaging.ACS applied nano materials · 2022Article
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- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Wide-field optical spectroscopy system integrating reflectance and spatial frequency domain imaging to measure attenuation-corrected intrinsic tissue fluorescence in radical prostatectomy specimens.Biomedical optics express · 2020Article
- Auto-Phase-Locked Time-Resolved Luminescence Detection: Principles, Applications, and Prospects.Frontiers in chemistry · 2020Review
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- Mn Doped AZIS/ZnS Nanocrystals (NCs): Effects of Ag and Mn Levels on NC Optical Properties.Journal of alloys and compounds · 2018Article
- AIE-active polyanetholesulfonic acid sodium salts with room-temperature phosphorescence characteristics for FeRSC advances · 2018Article
- Ultrasensitive optical imaging with lanthanide lumiphores.Nature chemical biology · 2018Article
- Time Gated Luminescence Imaging of Immunolabeled Human Tissues.Analytical chemistry · 2017Article
- Mn Doped AIZS/ZnS Nanocrystals: Synthesis and Optical Properties.Journal of alloys and compounds · 2017Article
- A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors.Lab on a chip · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An optical microscope capable of measuring time resolved luminescence (phosphorescence and delayed fluorescence) images has been developed. The technique employs two phase-locked mechanical choppers and a slow-scan scientific CCD camera attached to a normal fluorescence microscope. The sample is illuminated by a periodic train of light pulses and the image is recorded within a defined time interval after the end of each excitation period. The time resolution discriminates completely against light scattering, reflection, autofluorescence, and extraneous prompt fluorescence, which ordinarily decrease contrast in normal fluorescence microscopy measurements. Time resolved image microscopy produces a high contrast image and particular structures can be emphasized by displaying a new parameter, the ratio of the phosphorescence to fluorescence. Objects differing in luminescence decay rates are easily resolved. The lifetime of the long lived luminescence can be measured at each pixel of the microscope image by analyzing a series of images that differ by a variable time delay. The distribution of luminescence decay rates is displayed directly as an image. Several examples demonstrate the utility of the instrument and the complementarity it offers to conventional fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.