ArticleChromosoma1991
Nucleoli, nucleolar chromosomes and ribosomal genes in the human spermatocyte.
Article in Chromosoma, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- A working model for the formation of Robertsonian chromosomes.Journal of cell science · 2024Article
- Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum.Journal of experimental botany · 2019Article
- FBXO25-associated nuclear domains: a novel subnuclear structure.Molecular biology of the cell · 2008Article
- Additional nucleoli and NOR activity during meiotic prophase I in larch (Larix decidua Mill.).Protoplasma · 2007Article
- Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition.Molecular biology of the cell · 2005Article
- Nucleolonema as a fundamental substructure of the nucleolus.Journal of plant research · 2005Review
- Review
- Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Non-isotopic electron microscope in situ hybridization for studying the functional sub-compartmentalization of the cell nucleus.Histochemistry and cell biology · 1996Review
- Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes.The Histochemical journal · 1995Article
- Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids.Cell and tissue research · 1994Article
- A cytochemical study of the nucleolus and nucleolus-related structures during human spermatogenesis.Anatomy and embryology · 1994Article
- The nucleolus.Anatomy and embryology · 1993Review
- Three-dimensional organization of the ribosomal genes and Ag-NOR proteins during interphase and mitosis in PtK1 cells studied by confocal microscopy.Chromosoma · 1993Article
- Ultrastructural distribution of DNA within the nucleolus of various animal cell lines or tissues revealed by terminal deoxynucleotidyl transferase.Cell and tissue research · 1993Article
- New data concerning the functional organization of the mammalian cell nucleolus: detection of RNA and rRNA by in situ molecular immunocytochemistry.Nucleic acids research · 1992Article
- Nucleolar morphology and rDNA in situ hybridisation in monocytes.Cell and tissue research · 1992Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation and development of nucleoli and their connections with the nucleolar chromosomes were studied in human spermatocytes using electron microscopy, silver staining of nucleolus organizer regions (NORs), high resolution autoradiography and in situ hybridization in order to localize rRNA genes and their transcription in the different stages of meiotic prophase I. At leptotene, new nucleoli were formed, consisting of a fibrillar centre surrounded by a cap of dense fibrillar component. Following [3H]uridine uptake, label was found only over the dense fibrillar component. In situ hybridization revealed rDNA mainly in the dense fibrillar component and in the chromatin. During zygotene, nucleoli increased in size. The fibrillar centre was connected with the secondary constriction region of the nucleolar bivalent and was partially surrounded by dense fibrillar component. This shell of dense fibrillar component merged into a fibrillo-granular mesh that extended away from the fibrillar centre. Autoradiography following [3H]uridine uptake again showed the label overlaying the dense fibrillar component and the proximal part of the fibrillo-granular strands. With in situ hybridization in both the light and electron microscope, signal was mainly found in the dense fibrillar component. A small quantity of label was observed in the peripheral region of the fibrillar centre and in the adjacent chromatin. From early to late pachytene segregation of nucleolar components occurred, with a reduction in the dense fibrillar component that formed a narrow rim around the fibrillar centre with small extensions along the granular component. [3H]uridine incorporation progressively decreased. In situ hybridization showed signal located mainly in the dense fibrillar component and in the chromatin corresponding to the condensed short arm of the nucleolar bivalent. Our results indicate that the majority of rDNA is located and transcribed in the dense fibrillar component; only a small amount is present in the peripheral part of the fibrillar centre and may be transcribed there. Moreover, from leptotene to zygotene, rDNA unravels from the nucleolar chromosome into the nucleolar dense fibrillar component. From zygotene to late pachytene a progressive return to the condensed acrocentric short arm is observed.
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