ArticleJournal of clinical microbiology2007
Human papillomavirus quantification in urine and cervical samples by using the Mx4000 and LightCycler general real-time PCR systems.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Accuracy of urinary human papillomavirus testing for presence of cervical HPV: systematic review and meta-analysis.BMJ (Clinical research ed.) · 2014Pooled it
- Negative Xpert HPV Test (Cepheid) With Late Amplification Signals: Is There a Clinical Significance? (the PaPCR Study).Journal of medical virology · 2026Article
- Article
- Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine.European journal of medical research · 2024Article
- Comparison of Urine and Genital Samples for Detecting Human Papillomavirus (HPV) in Clinical Patients.Obstetrics and gynecology international · 2023Article
- Analysis of HR-HPV Infection Concordance Rates in Cervical and Urine Specimens; Proposal of Additional Cervical Screening Process for Women Who Refuse Invasive Cervical Sampling.Journal of personalized medicine · 2022Article
- Study Protocol: Randomised Controlled Trial Assessing the Efficacy of Strategies Involving Self-Sampling in Cervical Cancer Screening.International journal of public health · 2022Article
- The preference of women living with HIV for the HPV self-sampling of urine at a rural HIV clinic in Uganda.Southern African journal of infectious diseases · 2022Article
- Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine.Molecules (Basel, Switzerland) · 2021Article
- Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen.Microorganisms · 2020Article
- Quadruplex real-time PCR for rapid detection of human alphaherpesviruses.Medical microbiology and immunology · 2019Article
- Cervical cancer detection by DNA methylation analysis in urine.Scientific reports · 2019Article
- Detection of urinary Chlamydia trachomatis, Mycoplasma genitalium and human papilloma virus in the first trimester of pregnancy by PCR method.Annals of clinical microbiology and antimicrobials · 2018Article
- Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing.International journal of molecular sciences · 2016Article
- Comparison of Human Papillomavirus Detection in Urine and Cervical Samples Using High-Risk HPV DNA Testing in Northern Thailand.Obstetrics and gynecology international · 2016Article
- Optimization of HPV DNA detection in urine by improving collection, storage, and extraction.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Urine-based human papillomavirus DNA testing as a screening tool for cervical cancer in high-risk women.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2014Article
- Comparison of human papillomavirus detections in urine, vulvar, and cervical samples from women attending a colposcopy clinic.Journal of clinical microbiology · 2014Article
- Monitoring human papillomavirus prevalence in urine samples: a review.Clinical epidemiology · 2013Article
Corrections and comments
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Authors and funding
18 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the last decade, growing efforts have focused on human papillomavirus (HPV) detection using liquid hybridization, conventional PCR, and real-time PCR-based methods to increase the overall proportion of patients participating in cervical cancer screening procedures. We proposed a new general HPV DNA real-time PCR on the Mx4000 (Stratagene) and LightCycler (Roche Diagnostics) systems usable for both cervical scrape specimens and urine samples. A linear range was obtained from 5 DNA copies to 8 log(10) DNA copies/ml, and intra- and interassay variations were between 1.8 and 4%. Cervical carcinoma and HPV DNA screening was performed in 333 individual women referred for gynecological examination at the university hospitals of Angers and Brest and enrolled in the PapU study. Among cervical specimens (n = 333), 45% were positive for HPV DNA, with a mean viral load at 5.00 log/ml (+/- 1.73). Among urine samples (n = 177), 37% were positive with a significant 50-fold-lower mean viral load (3.77 +/- 1.32 log/ml; P < 0.0001). Kappa agreement for HPV DNA between cervical and urine specimens was excellent (93%). Thus, we developed a highly sensitive and quantitative general HPV DNA real-time PCR method that allows mass screening of patients with HPV infection. The ongoing longitudinal and prospective multicenter PapU study should give us the opportunity to validate this method adapted to HPV DNA screening in urine samples in a larger population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.