ArticleJournal of clinical microbiology2007
Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- False-negative CMV PCR results due to viral sequence variation: a diagnostic pitfall with the potential for serious consequences.ASM case reports · 2026Article
- An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5.Veterinary sciences · 2023Article
- Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea.Animals : an open access journal from MDPI · 2023Article
- Designing sensitive viral diagnostics with machine learning.Nature biotechnology · 2022Article
- In silico re-assessment of a diagnostic RT-qPCR assay for universal detection of Influenza A viruses.Scientific reports · 2019Article
- Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay.Scientific reports · 2017Article
- Clinical significance of low cytomegalovirus DNA levels in human plasma.Journal of clinical microbiology · 2012Article
- Diagnostic consequences of cytomegalovirus glycoprotein B polymorphisms.Journal of clinical microbiology · 2011Article
- Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis.Journal of clinical microbiology · 2010Article
- International standards and reference materials for quantitative molecular infectious disease testing.The Journal of molecular diagnostics : JMD · 2010Review
- Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays.Journal of clinical microbiology · 2008Article
- Exon 4 of the human cytomegalovirus (CMV) major immediate-early gene as a target for CMV real-time PCR.Journal of clinical microbiology · 2008Article
Corrections and comments
- Commented on by
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report how variability in the human cytomegalovirus genome sequence may seriously affect the outcome of its molecular diagnosis. A real-time quantitative PCR assay targeting the major immediate-early gene failed to detect the viral load in some, but not all, clinical samples from hematooncological patients. By amplification and sequencing the DNA across the regions targeted by this assay we found a number of nucleotide substitutions which accounted for decreased primer/probe binding. This decreased the sensitivity of the assay up to 1,000-fold.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.