ArticleGut2007
G protein-coupled receptor kinase 6 controls chronicity and severity of dextran sodium sulphate-induced colitis in mice.
Article in Gut, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Systematic review of animal models of post-infectious/post-inflammatory irritable bowel syndrome.Journal of gastroenterology · 2011Pooled it
- Untargeted Metabolomics Analysis of Lactic Acid Bacteria FermentedMolecules (Basel, Switzerland) · 2024Article
- The Role of G Protein-Coupled Receptor Kinase 6 Regulation in Inflammation and Pain.International journal of molecular sciences · 2022Review
- The GRKs Reactome: Role in Cell Biology and Pathology.International journal of molecular sciences · 2021Review
- MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6.Molecular and cellular biochemistry · 2019Article
- Article
- G protein-coupled receptor kinase-2-deficient mice are protected from dextran sodium sulfate-induced acute colitis.Physiological genomics · 2018Article
- Role of G protein-coupled receptor kinase-6 inPhysiological genomics · 2017Article
- Proinflammatory Cytokines Mediate GPCR Dysfunction.Journal of cardiovascular pharmacology · 2017Review
- G-protein-coupled receptor kinase-2 is a critical regulator of TNFα signaling in colon epithelial cells.The Biochemical journal · 2017Article
- G Protein-Coupled Receptor Kinases in the Inflammatory Response and Signaling.Advances in immunology · 2017Review
- Prognostic value of decreased GRK6 expression in lung adenocarcinoma.Journal of cancer research and clinical oncology · 2016Article
- GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal.Cell death & disease · 2016Article
- Article
- G-protein-coupled receptor kinases in inflammation and disease.Genes and immunity · 2015Review
- Functional consequences of perturbed CXCL12 signal processing: analyses of immature hematopoiesis in GRK6-deficient mice.Stem cells and development · 2015Article
- Melatonin reduces ulcerative colitis-associated local and systemic damage in mice: investigation on possible mechanisms.Digestive diseases and sciences · 2013Article
- Decreased expression and role of GRK6 in spinal cord of rats after chronic constriction injury.Neurochemical research · 2013Article
- G protein-coupled receptor kinase 6 acts as a critical regulator of cytokine-induced hyperalgesia by promoting phosphatidylinositol 3-kinase and inhibiting p38 signaling.Molecular medicine (Cambridge, Mass.) · 2012Article
- G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.Pharmacology & therapeutics · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfiltration of inflammatory cells into the colon plays an important role in the onset and course of inflammatory bowel disease. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors, including those involved in the migration of inflammatory cells. Therefore, it is hypothesised that GRK6 plays a role in determining the course of inflammation.
aimTo analyse the role of GRK6 in the course of dextran sodium sulphate (DSS)-induced colitis.
methodsColitis was induced by administering 1% DSS in drinking water to GRK6(-/-), GRK6(+/-) and wild-type (WT) mice for 6 days. The severity of colitis was assessed on the basis of clinical signs, colon length and histology. Moreover, keratinocyte-derived chemokine (KC) levels, granulocyte infiltration, interleukin 1beta (IL1beta), CD4, CD8 and forkhead box protein P3 (FoxP3) expression in the colon were determined. In addition, regulatory T cell function in WT and GRK6(-/-) mice was analysed. The chemotactic response of granulocytes to colon culture supernatants was assessed using a transendothelial migration assay.
resultsThe severity of colitis was increased in GRK6(-/-) and GRK6(+/-) mice and was accompanied by increased KC levels and increased granulocyte infiltration. Moreover, the chemotactic response of GRK6(-/-) granulocytes to supernatants of colon cultures was enhanced. Interestingly, the WT mice completely recovered from colitis, whereas the GRK6(-/-) and GRK6(+/-) mice developed chronic colitis, which was accompanied by increased IL1beta and CD4 expression and decreased FoxP3 expression. Moreover, regulatory T cell function was impaired in the GRK6(-/-) mice.
conclusionsThe intracellular level of GRK6 is an important factor in determining the onset, severity and chronicity of DSS-induced colitis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.