ArticleNeural development2007
Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
Article in Neural development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Of travelling homeoproteins and medulloblastomas.Oncogene · 2025Pooled it
- First pilot study of intrastromal rAAV-PAX6 gene therapy suggests improved corneal thickness and transcription correction in aniridic mouse.Molecular therapy. Advances · 2026Article
- Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish.Communications biology · 2025Article
- Therapeutic value of homeoprotein signaling pathways.Frontiers in neuroscience · 2024Review
- ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneurons.EMBO reports · 2023Article
- A Computational Approach to Explaining Bioelectrically Induced Persistent, Stochastic Changes of Axial Polarity in Planarian Regeneration.Bioelectricity · 2022Article
- Shuttling Homeoproteins and Their Biological Significance.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Genetic Regulation of Vertebrate Forebrain Development by Homeobox Genes.Frontiers in neuroscience · 2022Review
- HOX Protein Activity Regulation by Cellular Localization.Journal of developmental biology · 2021Review
- OTX2 signaling in retinal dysfunction, degeneration and regeneration.Neural regeneration research · 2021Article
- Homeoprotein transduction in neurodevelopment and physiopathology.Science advances · 2020Review
- Article
- Reflections on the use of protein binders to study protein function in developmental biology.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Bifacial stem cell niches in fish and plants.Current opinion in genetics & development · 2017Review
- Article
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- A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission.Scientific reports · 2016Article
- Dissecting the role of Engrailed in adult dopaminergic neurons--Insights into Parkinson disease pathogenesis.FEBS letters · 2015Review
- The bHLH Transcription Factor NeuroD Governs Photoreceptor Genesis and Regeneration Through Delta-Notch Signaling.Investigative ophthalmology & visual science · 2015Article
- Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain.Development (Cambridge, England) · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundModifications in Pax6 homeogene expression produce strong eye phenotypes. This suggested to us that eye development might be an appropriate model to verify if homeoprotein intercellular passage has important functions in early development. Similar to other homeoproteins, Pax6 has two domains that enable secretion and internalization by live cells and, thus, intercellular passage. In principle, a straightforward way to test the hypothesis would be to mutate one of the two sequences to produce a 'cell autonomous only' Pax6. However, this was not possible because these sequences are in the homeodomain and their modification would affect Pax6 transcriptional properties. We have thus developed an approach aimed at blocking Pax6 only in the extracellular milieu of developing zebrafish embryos.
resultsA first strategy was to inject a one-cell embryo with a mRNA encoding a secreted single-chain anti-Pax6 antibody. A second, complementary, strategy was to inject a Pax6 antibody in the blastula extracellular milieu. In both cases, 'dissymmetric eyes', 'one eye only' and 'no eye' phenotypes were produced. In most cases, lens phenotypes paralleled retina malformations. Although eye phenotypes were analyzed 30 hours post-fertilization, there was a strong correlation between early eye field asymmetry, early asymmetry in Pax6 expression and later-occurring eye malformations. Several controls were introduced, demonstrating that the effect is specific to Pax6 and cannot be explained by intracellular antibody activities.
conclusionThis study supports the hypothesis that the Pax6 transcription factor is also a signaling molecule with direct non-cell autonomous activity.
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