ArticleWorld journal of gastroenterology2007
Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients.
Article in World journal of gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03979339 (Feasibility of a New Technology for Isolating Circulating Tumour Cells in Selected Cancer Patients and Healthy Volunteers), which is not on this map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Feasibility of a New Technology for Isolating Circulating Tumour Cells in Selected Cancer Patients and Healthy Volunteers
Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer.EBioMedicine · 2016Trial
- Trial
- The Prognostic Significance of Circulating Tumor Cells in Patients with Pancreatobiliary Cancer.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2023Article
- Epithelial to Mesenchymal Transition as Mechanism of Progression of Pancreatic Cancer: From Mice to Men.Cancers · 2022Review
- Prognostic value of disseminated tumor cells in unresectable pancreatic ductal adenocarcinoma: a prospective observational study.BMC cancer · 2022Observational
- Exploring the Clinical Utility of Pancreatic Cancer Circulating Tumor Cells.International journal of molecular sciences · 2022Review
- Article
- Review
- Circulating Tumor Cells Predict Occult Metastatic Disease and Prognosis in Pancreatic Cancer.Annals of surgical oncology · 2018Article
- Circulating and disseminated tumor cells in pancreatic cancer and their role in patient prognosis: a systematic review and meta-analysis.Oncotarget · 2017Article
- No-touch isolation techniques for pancreatic cancer.Surgery today · 2017Review
- Liquid biopsy in patients with pancreatic cancer: Circulating tumor cells and cell-free nucleic acids.World journal of gastroenterology · 2016Review
- Clinical applications of circulating tumor DNA and circulating tumor cells in pancreatic cancer.Molecular oncology · 2016Review
- Diagnostic utility of clinical and biochemical parameters in pancreatic head malignancy patients with normal carbohydrate antigen 19-9 levels.African health sciences · 2015Article
- Improving pancreatic cancer diagnosis using circulating tumor cells: prospects for staging and single-cell analysis.Expert review of molecular diagnostics · 2015Review
- Current Status of CTCs as Liquid Biopsy in Lung Cancer and Future Directions.Frontiers in oncology · 2015Review
- Circulating tumor cells in the diagnosis and management of pancreatic cancer.Biochimica et biophysica acta · 2012Review
- Advances in biomarker research for pancreatic cancer.Current pharmaceutical design · 2012Review
- Diagnostic and prognostic biomarkers in pancreatic carcinoma.International journal of clinical and experimental pathology · 2009Article
- Analysis of microRNAs in pancreatic fine-needle aspirates can classify benign and malignant tissues.Clinical chemistry · 2008Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the diagnostic potential of cytokeratin-19 (CK-19) mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas.
methodsSixty-eight patients with pancreatic cancer (n = 37), chronic pancreatitis (n = 16), and non-pancreatic benign surgical diseases (n = 15, control group) were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR.
resultsCK-19 mRNA expression was increased in 24 (64%) blood samples and 11 (30%) of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 (40%) of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors (G2/G3). Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis.
conclusionDisseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinoma by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.
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