ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2007
Alterations of amino acids and glutamate transport in the DBA/2J mouse retina; possible clues to degeneration.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Metabolomics in Primary Open Angle Glaucoma: A Systematic Review and Meta-Analysis.Frontiers in neuroscience · 2022Pooled it
- Molecular pathways in experimental glaucoma models.Frontiers in neuroscience · 2024Review
- Review
- Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma.Chinese medicine · 2021Article
- Tryptophan Pathway Abnormalities in a Murine Model of Hereditary Glaucoma.International journal of molecular sciences · 2021Article
- Tryptophan and Kynurenine Pathway Metabolites in Animal Models of Retinal and Optic Nerve Damage: Different Dynamics of Changes.Frontiers in physiology · 2019Article
- Arundic acid attenuates retinal ganglion cell death by increasing glutamate/aspartate transporter expression in a model of normal tension glaucoma.Cell death & disease · 2015Article
- Loss of outer retinal neurons and circuitry alterations in the DBA/2J mouse.Investigative ophthalmology & visual science · 2014Article
- Down-regulation of the RNA editing enzyme ADAR2 contributes to RGC death in a mouse model of glaucoma.PloS one · 2014Article
- Hyperbaric pressure and increased susceptibility to glutamate toxicity in retinal ganglion cells in vitro.Molecular vision · 2014Article
- Protective effect of oxysophoridine on cerebral ischemia/reperfusion injury in mice.Neural regeneration research · 2013Article
- Enhanced expression of NR2B subunits of NMDA receptors in the inherited glaucomatous DBA/2J mouse retina.Neural plasticity · 2013Article
- Review
- Impairment of neuronal glutamate uptake and modulation of the glutamate transporter GLT-1 induced by retinal ischemia.PloS one · 2013Article
- Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model.Investigative ophthalmology & visual science · 2011Article
- Investigation of the association between SLC1A3 gene polymorphisms and normal tension glaucoma.Molecular vision · 2011Article
- Differential expression of two glutamate transporters, GLAST and GLT-1, in an experimental rat model of glaucoma.Experimental brain research · 2009Article
- K(+) currents fail to change in reactive retinal glial cells in a mouse model of glaucoma.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe DBA/2J mouse spontaneously develops ocular hypertension and time-dependent progressive retinal ganglion cell (RGC) loss. This study examines changes in amino acid levels in the vitreous, and changes in the expression of retinal glutamate transporters and receptors that occur during the progression of this pathology.
methodsRetinas were obtained from DBA/2J mice at ages 3, 6 and 11 months. C57BL/6 mice were used as age-matched controls. Vitreal amino acid content was measured with HPLC. Western blotting and immunohistochemistry were performed using specific antibodies against the glutamate transporters (GLAST, GLT-1v, EAAC-1) and glutamate receptors, particularly NMDA (NR1, NR2A, NR2B) and AMPA (GluR1, GluR2/3, GluR4) receptors.
resultsHPLC showed retinal concentrations of glutamate, glutamine, glycine, alanine, lysine, serine, and arginine to be significantly higher in DBA/2J mice at 11 months of age compared to age-matched controls. Western Blots revealed a moderate decrease of GLAST and GLT-1v expression in DBA/2J mice at 6 and 11 months as compared to age-matched controls while there was no change in EAAC1. Immunohistochemically, no changes in expression of NMDA and AMPA receptors were seen.
conclusionAlterations of amino acid content and enhanced glutamate neurotransmission might be involved in the pathogenesis of retinal neurodegeneration in the DBA/ 2J mouse model of ocular hypertension. Moreover, these mice provide an animal model for studying excitotoxic retinal damage.
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