ArticleMechanisms of ageing and development2007
WRN exonuclease activity is blocked by DNA termini harboring 3' obstructive groups.
Article in Mechanisms of ageing and development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- The DinG exonuclease acts as a primary quality controller to remove unprocessed ribosomal RNAs.Nucleic acids research · 2026Article
- Response to Replication Stress and Maintenance of Genome Stability by WRN, the Werner Syndrome Protein.International journal of molecular sciences · 2024Review
- TREX1 degrades the 3' end of the small DNA oligonucleotide products of nucleotide excision repair in human cells.Nucleic acids research · 2022Article
- Human TDP1, APE1 and TREX1 repair 3'-DNA-peptide/protein cross-links arising from abasic sites in vitro.Nucleic acids research · 2022Article
- Perspectives on formaldehyde dysregulation: Mitochondrial DNA damage and repair in mammalian cells.DNA repair · 2021Review
- TREX1 - Apex predator of cytosolic DNA metabolism.DNA repair · 2020Article
- Evidence for premature aging in a Drosophila model of Werner syndrome.Experimental gerontology · 2019Article
- Measuring TREX1 and TREX2 exonuclease activities.Methods in enzymology · 2019Article
- Review
- APE1 is dispensable for S-region cleavage but required for its repair in class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers.The Journal of biological chemistry · 2014Article
- Human RecQ helicases in DNA repair, recombination, and replication.Annual review of biochemistry · 2014Review
- The Drosophila orthologue of progeroid human WRN exonuclease, DmWRNexo, cleaves replication substrates but is inhibited by uracil or abasic sites : analysis of DmWRNexo activity in vitro.Age (Dordrecht, Netherlands) · 2013Article
- Processing of damaged DNA ends for double-strand break repair in mammalian cells.ISRN molecular biology · 2012Article
- DNA repair deficiency in neurodegeneration.Progress in neurobiology · 2011Review
- Roles of Werner syndrome protein in protection of genome integrity.DNA repair · 2010Review
- Yeast Tdp1 regulates the fidelity of nonhomologous end joining.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Tyrosyl-DNA phosphodiesterase and the repair of 3'-phosphoglycolate-terminated DNA double-strand breaks.DNA repair · 2009Article
- Rising from the RecQ-age: the role of human RecQ helicases in genome maintenance.Trends in biochemical sciences · 2008Review
- The clinical characteristics of Werner syndrome: molecular and biochemical diagnosis.Human genetics · 2008Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Reactive oxygen species, generated either by cellular respiration or upon exposure to environmental agents such as ionizing radiation (IR), attack DNA to form a variety of oxidized base and sugar modifications. Accumulation of oxidative DNA damage has been associated with age-related disease as well as the aging process. Single-strand breaks harboring oxidative 3' obstructive termini, e.g. 3' phosphates and 3' phosphoglycolates, must be removed prior to DNA repair synthesis or ligation. In addition, 3' tyrosyl-linked protein damage, resulting from therapeutic agents such as camptothecin (CPT), must be processed to initiate repair. Several nucleases participate in DNA repair and the excision of 3' obstructive ends. As the protein defective in the segmental progeroid Werner syndrome (WRN) possesses 3'-5' exonuclease activity, and Werner syndrome cells are hypersensitive to IR and CPT, we examined for WRN exonuclease activity on 3' blocking lesions. Moreover, we compared side-by-side the activity of four prominent human 3'-5' exonucleases (WRN, APE1, TREX1, and p53) on substrates containing 3' phosphates, phosphoglycolates, and tyrosyl residues. Our studies reveal that while WRN degrades 3' hydroxyl containing substrates in a non-processive manner, it does not excise 3' phosphate, phosphoglycolate, or tyrosyl groups. In addition, we found that APE1 was most active at excising 3' blocking termini in comparison to the disease-related exonucleases TREX1, WRN, and p53 under identical physiological reaction conditions, and that TREX1 was the most powerful 3'-5' exonuclease on undamaged oligonucleotide substrates.
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