ArticlePlanta2007
Characterization of a stress responsive proteinase inhibitor gene with positive effect in improving drought resistance in rice.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 115 citations in OpenAlex.
- Temporal transcriptome and WGCNA analysis unveils divergent drought response strategies in wild and cultivatedFrontiers in plant science · 2025Article
- Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in kumquat.Horticulture research · 2023Article
- The roles of plant proteases and protease inhibitors in drought response: a review.Frontiers in plant science · 2023Review
- Omics: a tool for resilient rice genetic improvement strategies.Molecular biology reports · 2022Review
- Physiological and Molecular Responses of 'Dusa' Avocado Rootstock to Water Stress: Insights for Drought Adaptation.Plants (Basel, Switzerland) · 2021Article
- Modulation of Abiotic Stress Responses in Rice by E3-Ubiquitin Ligases: A Promising Way to Develop Stress-Tolerant Crops.Frontiers in plant science · 2021Review
- Genome-Wide Identification and Expression Analysis of the Protease Inhibitor Gene Families in Tomato.Genes · 2019Article
- Evaluation of Glycosyl-Hydrolases, Phosphatases, Esterases and Proteases as Potential Biomarker for NaCl-Stress Tolerance inMolecules (Basel, Switzerland) · 2019Article
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- Expression analysis of proteinase inhibitor-II under3 Biotech · 2018Article
- Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.Plant biotechnology journal · 2017Article
- Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing.BMC genomics · 2017Article
- Expression of theFrontiers in plant science · 2017Article
- Molecular Characterization and Expression Profiling ofFrontiers in plant science · 2017Article
- Identification of drought stress related proteins from 1SBotanical studies · 2016Article
- Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes.Plant physiology · 2016Article
- Isolation and Functional Characterization of Bidirectional Promoters in Rice.Frontiers in plant science · 2016Article
- A stress-responsive NAC transcription factor SNAC3 confers heat and drought tolerance through modulation of reactive oxygen species in rice.Journal of experimental botany · 2015Article
- Functional Characterization of Cucumis metuliferus Proteinase Inhibitor Gene (CmSPI) in Potyviruses Resistance.Viruses · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A full-length cDNA gene, designated Oryza sativa chymotrypsin inhibitor-like 1 (OCPI1), was characterized in rice. The predicted protein of OCPI1 shows very high sequence identity to reported chymotrypsin inhibitors from various plant species. Northern-blot analysis showed that the expression of OCPI1 was strongly induced by dehydration stresses and abscisic acid (ABA). The expression of beta-glucuronidase (GUS) reporter gene under the control of OCPI1 promoter transformed into rice was strongly induced by drought and salt stresses. Interestingly, strong dehydration stress-induced GUS activity was also detected in the transgenic rice containing the reverse sequence of OCPI1 promoter fused to GUS gene, suggesting of a bidirectional transcriptional activity in the OCPI1 promoter. OCPI1 gene was over-expressed in japonica cv. Zhonghua 11 and transgenic plants containing single copy of transgene were tested for drought resistance at reproductive stage. The positive transgenic plants (OCPI1 was over-expressed) had significantly higher grain yield and seed setting rate than the wild type and the negative transgenic control (no over-expression of the transgene) under the severe drought stress conditions, whereas the potential yield of transgenic plants under normal growth conditions was not affected. Chymotrypsin-inhibitor activity assay showed that the crude protein of the positive transgenic plants had stronger inhibitory activity than the negative control. Transgenic plants had less decrease of total proteins than the wild type under drought stress. Taken together, these data indicate that OCPI1 might potentially be useful in the genetic improvement of drought resistance in rice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.