ArticleMolecular and cellular biology2007
Positive and negative regulation of Tetrahymena telomerase holoenzyme.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast.Nature communications · 2018Article
- Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo.Protoplasma · 2017Article
- Evolutionary perspectives of telomerase RNA structure and function.RNA biology · 2016Review
- The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.Nature structural & molecular biology · 2015Article
- Proteomics of yeast telomerase identified Cdc48-Npl4-Ufd1 and Ufd4 as regulators of Est1 and telomere length.Nature communications · 2015Article
- 21st Century Genetics: Mass Spectrometry of Yeast Telomerase.Cold Spring Harbor symposia on quantitative biology · 2015Article
- Direct single-stranded DNA binding by Teb1 mediates the recruitment of Tetrahymena thermophila telomerase to telomeres.Molecular and cellular biology · 2014Article
- Telomerase structure.Current opinion in structural biology · 2014Review
- Progress in structural studies of telomerase.Current opinion in structural biology · 2014Review
- Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub.Molecular and cellular biology · 2013Article
- Biogenesis of telomerase ribonucleoproteins.RNA (New York, N.Y.) · 2012Review
- Roles of telomerase reverse transcriptase N-terminal domain in assembly and activity of Tetrahymena telomerase holoenzyme.The Journal of biological chemistry · 2012Article
- Telomerase and telomere-associated proteins: structural insights into mechanism and evolution.Structure (London, England : 1993) · 2012Review
- Article
- Multiple mechanisms for elongation processivity within the reconstituted tetrahymena telomerase holoenzyme.The Journal of biological chemistry · 2010Article
- An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenance.Molecular cell · 2009Article
- Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila.Genes & development · 2009Article
- A single RNA-dependent RNA polymerase assembles with mutually exclusive nucleotidyl transferase subunits to direct different pathways of small RNA biogenesis.RNA (New York, N.Y.) · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Telomerase replenishes the telomeric repeats that cap eukaryotic chromosome ends. To perform DNA synthesis, the active site of telomerase reverse transcriptase (TERT) copies a template within the integral telomerase RNA (TER). In vivo, TERT and TER and additional subunits form a telomerase holoenzyme capable of telomere elongation. We previously purified epitope-tagged Tetrahymena thermophila TERT and characterized two of the associated proteins. Here we characterize the remaining two proteins that were enriched by TERT purification. The primary sequence of the p75 polypeptide lacks evident homology with other proteins, whereas the p20 polypeptide is the Tetrahymena ortholog of a conserved multifunctional protein, Skp1. Genetic depletion of p75 induced telomere shortening without affecting the accumulation of TER or TERT, suggesting that p75 promotes telomerase function at the telomere. Affinity purification of p75 coenriched telomerase activity and each other known telomerase holoenzyme protein. On the other hand, genetic depletion of Skp1p induced telomere elongation, suggesting that this protein plays a negative regulatory role in the maintenance of telomere length homeostasis. Affinity purification of Skp1p did not detectably enrich active telomerase but did copurify ubiquitin ligase machinery. These studies reveal additional complexity in the positive and negative regulation of Tetrahymena telomerase function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.