ArticleApplied and environmental microbiology2007
Detection of Vibrio cholerae by real-time nucleic acid sequence-based amplification.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 85 citations in OpenAlex.
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- Impact of a human gut microbe oneLife · 2022Article
- A Review of the Environmental Trigger and Transmission Components for Prediction of Cholera.Tropical medicine and infectious disease · 2021Review
- Population Structure and Multidrug Resistance of Non-O1/Non-O139 Vibrio cholerae in Freshwater Rivers in Zhejiang, China.Microbial ecology · 2021Article
- Simultaneous Quantification ofPathogens (Basel, Switzerland) · 2020Article
- Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection.Cell · 2020Article
- Environmental and Clinical Strains ofFrontiers in microbiology · 2019Article
- Development of a real-time nucleic acid sequence-based amplification assay for the rapid detection of Salmonella spp. from food.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Point-of-care microfluidic devices for pathogen detection.Biosensors & bioelectronics · 2018Review
- Occurrence of VibrioItalian journal of food safety · 2016Article
- Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations.Frontiers in microbiology · 2014Review
- Cholera epidemiology in Nigeria: an overview.The Pan African medical journal · 2012Review
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
- Detection of Aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real-time nucleic acid sequence-based amplification.Journal of clinical microbiology · 2010Article
- Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections.Journal of clinical microbiology · 2009Article
- Detection of live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse transcriptase real-time PCR targeting invA mRNA.Applied and environmental microbiology · 2009Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multitarget molecular beacon-based real-time nucleic acid sequence-based amplification (NASBA) assay for the specific detection of Vibrio cholerae has been developed. The genes encoding the cholera toxin (ctxA), the toxin-coregulated pilus (tcpA; colonization factor), the ctxA toxin regulator (toxR), hemolysin (hlyA), and the 60-kDa chaperonin product (groEL) were selected as target sequences for detection. The beacons for the five different genetic targets were evaluated by serial dilution of RNA from V. cholerae cells. RNase treatment of the nucleic acids eliminated all NASBA, whereas DNase treatment had no effect, showing that RNA and not DNA was amplified. The specificity of the assay was investigated by testing several isolates of V. cholerae, other Vibrio species, and Bacillus cereus, Salmonella enterica, and Escherichia coli strains. The toxR, groEL, and hlyA beacons identified all V. cholerae isolates, whereas the ctxA and tcpA beacons identified the O1 toxigenic clinical isolates. The NASBA assay detected V. cholerae at 50 CFU/ml by using the general marker groEL and tcpA that specifically indicates toxigenic strains. A correlation between cell viability and NASBA was demonstrated for the ctxA, toxR, and hlyA targets. RNA isolated from different environmental water samples spiked with V. cholerae was specifically detected by NASBA. These results indicate that NASBA can be used in the rapid detection of V. cholerae from various environmental water samples. This method has a strong potential for detecting toxigenic strains by using the tcpA and ctxA markers. The entire assay including RNA extraction and NASBA was completed within 3 h.
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