ArticleApplied and environmental microbiology2007
Redirection of metabolism for biological hydrogen production.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 164 citations in OpenAlex.
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- Rice N-biofertilization by inoculation with an engineered photosynthetic diazotroph.World journal of microbiology & biotechnology · 2024Article
- Enhanced extracellular ammonium release in the plant endophyteMicrobiology spectrum · 2024Article
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- Photosynthetic biohybrid coculture for tandem and tunable COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Red Fluorescent Protein Reporter System Developed for Measuring Gene Expression in Photosynthetic Bacteria under Anaerobic Conditions.Microorganisms · 2022Article
- A directed genome evolution method to enhance hydrogen production inFrontiers in microbiology · 2022Article
- Characteristics and Application ofFrontiers in bioengineering and biotechnology · 2022Review
- Potential of Phototrophic Purple Nonsulfur Bacteria to Fix Nitrogen in Rice Fields.Microorganisms · 2021Review
- Effect of silica-core gold-shell nanoparticles on the kinetics of biohydrogen production and pollutant hydrogenation via organic acid photofermentation over enhanced near-infrared illumination.International journal of hydrogen energy · 2021Article
- The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producerInternational journal of hydrogen energy · 2020Article
- Reduction of Substrates by Nitrogenases.Chemical reviews · 2020Review
- Control of nitrogen fixation in bacteria that associate with cereals.Nature microbiology · 2020Article
- Engineering Photosynthetic Bioprocesses for Sustainable Chemical Production: A Review.Frontiers in bioengineering and biotechnology · 2020Review
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- Phototrophic Lactate Utilization byApplied and environmental microbiology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major route for hydrogen production by purple photosynthetic bacteria is biological nitrogen fixation. Nitrogenases reduce atmospheric nitrogen to ammonia with the concomitant obligate production of molecular hydrogen. However, hydrogen production in the context of nitrogen fixation is a rather inefficient process because about 75% of the reductant consumed by the nitrogenase is used to generate ammonia. In this study we describe a selection strategy to isolate strains of purple photosynthetic bacteria in which hydrogen production is necessary for growth and independent of nitrogen fixation. We obtained four mutant strains of the photosynthetic bacterium Rhodopseudomonas palustris that produce hydrogen constitutively, even in the presence of ammonium, a condition where wild-type cells do not accumulate detectable amounts of hydrogen. Some of these strains produced up to five times more hydrogen than did wild-type cells growing under nitrogen-fixing conditions. Transcriptome analyses of the hydrogen-producing mutant strains revealed that in addition to the nitrogenase genes, 18 other genes are potentially required to produce hydrogen. The mutations that caused constitutive hydrogen production mapped to four different sites in the NifA transcriptional regulator in the four different strains. The strategy presented here can be applied to the large number of diverse species of anoxygenic photosynthetic bacteria that are known to exist in nature to identify strains for which there are fitness incentives to produce hydrogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.