ArticleJournal of bacteriology2007
The HP0165-HP0166 two-component system (ArsRS) regulates acid-induced expression of HP1186 alpha-carbonic anhydrase in Helicobacter pylori by activating the pH-dependent promoter.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 61 citations in OpenAlex.
- Effect ofImmunotherapy · 2025Review
- Molecular insights into the fine-tuning of pH-dependent ArsR-mediated regulation of the SabA adhesin in Helicobacter pylori.Nucleic acids research · 2024Article
- Review
- Lactobacilli Downregulate Transcription Factors inInternational journal of molecular sciences · 2022Article
- Two-component regulatory systems inFrontiers in cellular and infection microbiology · 2022Review
- The Efficacy of Cancer Immunotherapies Is Compromised byFrontiers in immunology · 2022Review
- Bacterial two-component systems as sensors for synthetic biology applications.Current opinion in systems biology · 2021Article
- Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System.Infection and immunity · 2021Article
- The enigma of environmental pH sensing in plants.Nature plants · 2021Review
- Small Molecule Inhibitors of the Response Regulator ArsR Exhibit Bactericidal Activity againstMicroorganisms · 2020Article
- Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- A Novel View of HumanBiomolecules · 2019Review
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Measurement of Internal pH in Helicobacter pylori by Using Green Fluorescent Protein Fluorimetry.Journal of bacteriology · 2018Article
- High-Salt Conditions Alter Transcription of Helicobacter pylori Genes Encoding Outer Membrane Proteins.Infection and immunity · 2018Article
- Acidic pH triggers the phosphorylation of the response regulator NtrX in alphaproteobacteria.PloS one · 2018Article
- Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes.Nature communications · 2017Article
- Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation.Journal of bacteriology · 2016Article
- Use of VacA as a Vaccine Antigen.Toxins · 2016Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The periplasmic alpha-carbonic anhydrase of Helicobacter pylori is essential for buffering the periplasm at acidic pH. This enzyme is an integral component of the acid acclimation response that allows this neutralophile to colonize the stomach. Transcription of the HP1186 alpha-carbonic anhydrase gene is upregulated in response to low environmental pH. A binding site for the HP0166 response regulator (ArsR) has been identified in the promoter region of the HP1186 gene. To investigate the mechanism that regulates the expression of HP1186 in response to low pH and the role of the HP0165-HP0166 two-component system (ArsRS) in this acid-inducible regulation, Northern blot analysis was performed with RNAs isolated from two different wild-type H. pylori strains (26695 and 43504) and mutants with HP0165 histidine kinase (ArsS) deletions, after exposure to either neutral pH or low pH (pH 4.5). ArsS-dependent upregulation of HP1186 alpha-carbonic anhydrase in response to low pH was found in both strains. Western blot analysis of H. pylori membrane proteins confirmed the regulatory role of ArsS in HP1186 expression in response to low pH. Analysis of the HP1186 promoter region revealed two possible transcription start points (TSP1 and TSP2) located 43 and 11 bp 5' of the ATG start codon, respectively, suggesting that there are two promoters transcribing the HP1186 gene. Quantitative primer extension analysis showed that the promoter from TSP1 (43 bp 5' of the ATG start codon) is a pH-dependent promoter and is regulated by ArsRS in combating environmental acidity, whereas the promoter from TSP2 may be responsible for control of the basal transcription of HP1186 alpha-carbonic anhydrase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.