ArticleJournal of bacteriology2007
An unusual response regulator influences sporulation at early and late stages in Streptomyces coelicolor.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Characterization of two co-regulated response regulators inJournal of bacteriology · 2025Article
- Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.Microbial cell factories · 2024Article
- Two-Component Systems ofInternational journal of molecular sciences · 2022Review
- c-di-GMP signalling and the regulation of developmental transitions in streptomycetes.Nature reviews. Microbiology · 2015Review
- Response regulator heterodimer formation controls a key stage in Streptomyces development.PLoS genetics · 2014Article
- Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences.FEMS microbiology reviews · 2014Review
- Identification of new developmentally regulated genes involved in Streptomyces coelicolor sporulation.BMC microbiology · 2013Article
- Molecular regulation of antibiotic biosynthesis in streptomyces.Microbiology and molecular biology reviews : MMBR · 2013Review
- Constitutive expression of ftsZ overrides the whi developmental genes to initiate sporulation of Streptomyces coelicolor.Antonie van Leeuwenhoek · 2012Article
- Signals and regulators that govern Streptomyces development.FEMS microbiology reviews · 2012Review
- Selective overproduction of the proteasome inhibitor salinosporamide A via precursor pathway regulation.Chemistry & biology · 2011Article
- An orphan histidine kinase, OhkA, regulates both secondary metabolism and morphological differentiation in Streptomyces coelicolor.Journal of bacteriology · 2011Article
- SCO3900, co-transcripted with three downstream genes, is involved in the differentiation of Streptomyces coelicolor.Current microbiology · 2010Article
- Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Streptomyces morphogenetics: dissecting differentiation in a filamentous bacterium.Nature reviews. Microbiology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
WhiI, a regulator required for efficient sporulation septation in the aerial mycelium of Streptomyces coelicolor, resembles response regulators of bacterial two-component systems but lacks some conserved features of typical phosphorylation pockets. Four amino acids of the abnormal "phosphorylation pocket" were changed by site-directed mutagenesis. Unlike whiI null mutations, these point mutations did not interfere with sporulation septation but had various effects on spore maturation. Transcriptome analysis was used to compare gene expression in the wild-type strain, a D27A mutant (pale gray spores), a D69E mutant (wild-type spores), and a null mutant (white aerial mycelium, no spores) (a new variant of PCR targeting was used to introduce the point mutations into the chromosomal copy of whiI). The results revealed 45 genes that were affected by the deletion of whiI. Many of these showed increased expression in the wild type at the time when aerial growth and development were taking place. About half of them showed reduced expression in the null mutant, and about half showed increased expression. Some, but not all, of these 45 genes were also affected by the D27A mutation, and a few were affected by the D69E mutation. The results were consistent with a model in which WhiI acts differently at sequential stages of development. Consideration of the functions of whiI-influenced genes provides some insights into the physiology of aerial hyphae. Mutation of seven whiI-influenced genes revealed that three of them play roles in spore maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.