ArticleTransgenic research2007
A passage through in vitro culture leads to efficient production of marker-free transgenic plants in Brassica juncea using the Cre-loxP system.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Review
- BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Development of transgenic Brassica juncea lines for reduced seed sinapine content by perturbing phenylpropanoid pathway genes.PloS one · 2017Article
- Strength, Stability, and cis-Motifs of In silico Identified Phloem-Specific Promoters in Brassica juncea (L.).Frontiers in plant science · 2016Article
- Development of selectable marker free, insect resistant, transgenic mustard (Brassica juncea) plants using Cre/lox mediated recombination.BMC biotechnology · 2013Article
- Utilization of PVX-Cre expression vector in potato.Transgenic research · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics.Journal of biosciences · 2011Article
- Robust circadian rhythms of gene expression in Brassica rapa tissue culture.Plant physiology · 2010Article
- An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.).Plant cell reports · 2010Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Developmentally regulated site-specific marker gene excision in transgenic B. napus plants.Plant cell reports · 2009Article
- Generation of selectable marker-free sheath blight resistant transgenic rice plants by efficient co-transformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain.Plant cell reports · 2008Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cre-loxP site-specific recombination system was deployed for removal of marker genes from Brassica juncea (Indian mustard). Excision frequencies, monitored by removal of nptII or gfp genes in F(1) plants of crosses between LOX and CRE lines, were high in quiescent, differentiated somatic tissues but extremely poor in the meristematic regions (and consequently the germinal cells) thus preventing identification and selection of marker-free transgenic events which are devoid of both the marker gene as well as the cre gene, in F(2) progeny. We show that a passage through in vitro culture of F(1 )leaf explants allows efficient development of marker-free transgenics in the F(2) generation addressing current limitations associated with efficient use of the Cre/loxP technology for marker gene removal.
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Registered trials
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