ArticleThe Journal of cell biology1991
Polarized and functional epithelia can form after the targeted inactivation of both mouse keratin 8 alleles.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis.Cellular and molecular life sciences : CMLS · 2020Article
- Filaments and phenotypes: cellular roles and orphan effects associated with mutations in cytoplasmic intermediate filament proteins.F1000Research · 2019Review
- Dissection of keratin network formation, turnover and reorganization in living murine embryos.Scientific reports · 2015Article
- Article
- Intermediate filaments: a historical perspective.Experimental cell research · 2007Review
- Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.The Journal of cell biology · 2005Article
- Keratin 8 protection of placental barrier function.The Journal of cell biology · 2003Article
- Cytokeratins 8 and 19 in the mouse placental development.The Journal of cell biology · 2000Article
- Desmoplakin is required early in development for assembly of desmosomes and cytoskeletal linkage.The Journal of cell biology · 1998Article
- Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions.The Journal of cell biology · 1998Article
- Targeted disruption of decorin leads to abnormal collagen fibril morphology and skin fragility.The Journal of cell biology · 1997Article
- Oncogenic regulation and function of keratins 8 and 18.Cancer metastasis reviews · 1996Review
- Intermediate filaments as dynamic structures.Cancer metastasis reviews · 1996Review
- Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly.The Journal of cell biology · 1996Article
- Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.The Journal of cell biology · 1995Article
- Continual assembly of half-desmosomal structures in the absence of cell contacts and their frustrated endocytosis: a coordinated Sisyphus cycle.The Journal of cell biology · 1995Article
- Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.The Journal of cell biology · 1995Article
- AP-1, ETS, and transcriptional silencers regulate retinoic acid-dependent induction of keratin 18 in embryonic cells.Molecular and cellular biology · 1994Article
- Intermediate filaments and disease: mutations that cripple cell strength.The Journal of cell biology · 1994Review
- Functional analysis of desmoplakin domains: specification of the interaction with keratin versus vimentin intermediate filament networks.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have tested the requirement of keratin intermediate filaments for the formation and function of a simple epithelium. We disrupted both alleles of the mouse keratin 8 (mK8) gene in embryonic stem cells, and subsequently analyzed the phenotype in developing embryoid bodies in suspension culture. After the inactivation of the mouse keratin 8 (mK8) gene by a targeted insertion, mK8 protein synthesis was undetectable. In the absence of mK8 its complementary partners mK18 and mK19 were unable to form filaments within differentiated cells. Surprisingly, these ES cells differentiate to both simple and cystic embryoid bodies with apparently normal epithelia. Ultrastructural analysis shows an apparently normal epithelium with microvilli on the apical membrane, tight junctions and desmosomes on the lateral membrane, and an underlying basal membrane. No significant differences in the synthesis or secretion of alpha 1-fetoprotein and laminin were observed between the mK8- or wild-type embryoid bodies. Our data show that mK8 is not required for simple epithelium formation of extraembryonic endoderm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.