ArticleBiophysical journal2007
Investigation of domain formation in sphingomyelin/cholesterol/POPC mixtures by fluorescence resonance energy transfer and Monte Carlo simulations.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 128 citations in OpenAlex.
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- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.bioRxiv : the preprint server for biology · 2026Article
- A Theoretical Basis for Nanodomains.The Journal of membrane biology · 2022Review
- Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope.Membranes · 2022Article
- Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature.Biophysical reviews · 2022Article
- Review
- Critical Phenomena in Plasma Membrane Organization and Function.Annual review of physical chemistry · 2021Review
- Lattice Models for Protein Organization throughout Thylakoid Membrane Stacks.Biophysical journal · 2020Article
- Serotonin Alters the Phase Equilibrium of a Ternary Mixture of Phospholipids and Cholesterol.Frontiers in physiology · 2020Article
- Anomalous inter-membrane cholesterol transport in fluid phase phosphoserine vesicles driven by headgroup ordered to disordered entropic transition.Chemistry and physics of lipids · 2019Article
- Parameterization of a coarse-grained model of cholesterol with point-dipole electrostatics.Journal of computer-aided molecular design · 2018Article
- FRET Detects the Size of Nanodomains for Coexisting Liquid-Disordered and Liquid-Ordered Phases.Biophysical journal · 2018Article
- Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins.Scientific reports · 2017Article
- Charge Distribution Fine-Tunes the Translocation of α-Helical Amphipathic Peptides across Membranes.Biophysical journal · 2016Article
- Effects of Leucin-Enkephalins on Surface Characteristics and Morphology of Model Membranes Composed of Raft-Forming Lipids.The Journal of membrane biology · 2016Article
- Glucosylceramide Reorganizes Cholesterol-Containing Domains in a Fluid Phospholipid Membrane.Biophysical journal · 2016Article
- Hexagonal Substructure and Hydrogen Bonding in Liquid-Ordered Phases Containing Palmitoyl Sphingomyelin.Biophysical journal · 2015Article
- Trace membrane additives affect lipid phases with distinct mechanisms: a modified Ising model.European biophysics journal : EBJ · 2015Article
- Differential effect of plant lipids on membrane organization: specificities of phytosphingolipids and phytosterols.The Journal of biological chemistry · 2015Article
- Computer simulations suggest a key role of membranous nanodomains in biliary lipid secretion.PLoS computational biology · 2015Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
We have recently proposed a phase diagram for mixtures of porcine brain sphingomyelin (BSM), cholesterol (Chol), and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) on the basis of kinetics of carboxyfluorescein efflux induced by the amphipathic peptide delta-lysin. Although that study indicated the existence of domains, phase separations in the micrometer scale have not been observed by fluorescence microscopy in BSM/Chol/POPC mixtures, though they have for some other sphingomyelins (SM). Here we examine the same BSM/Chol/POPC system by a combination of fluorescence resonance energy transfer (FRET) and Monte Carlo simulations. The results clearly demonstrate that domains are formed in this system. Comparison of the FRET experimental data with the computer simulations allows the estimate of lipid-lipid interaction Gibbs energies between SM/Chol, SM/POPC, and Chol/POPC. The latter two interactions are weakly repulsive, but the interaction between SM and Chol is favorable. Furthermore, those three unlike lipid interaction parameters between the three possible lipid pairs are sufficient for the existence of a closed loop in the ternary phase diagram, without the need to involve multibody interactions. The calculations also indicate that the largest POPC domains contain several thousand lipids, corresponding to linear sizes of the order of a few hundred nanometers.
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