ArticleJournal of structural biology2007
Analysis of the orientation of primary cilia in growth plate cartilage: a mathematical method based on multiphoton microscopical images.
Article in Journal of structural biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- From polarity to pathology: Decoding the role of cell orientation in osteoarthritis.Journal of orthopaedic translation · 2024Review
- Physiological and Pathophysiological Aspects of Primary Cilia-A Literature Review with View on Functional and Structural Relationships in Cartilage.International journal of molecular sciences · 2020Review
- Primary cilia are necessary for Prx1-expressing cells to contribute to postnatal skeletogenesis.Journal of cell science · 2018Article
- [Primary cilium acts as an oxygen sensor in PC12 cells].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2017Article
- Biological and Chemical Removal of Primary Cilia Affects Mechanical Activation of Chondrogenesis Markers in Chondroprogenitors and Hypertrophic Chondrocytes.International journal of molecular sciences · 2016Article
- Function and regulation of primary cilia and intraflagellar transport proteins in the skeleton.Annals of the New York Academy of Sciences · 2015Review
- Automated Cell Detection and Morphometry on Growth Plate Images of Mouse Bone.Applied mathematics · 2014Article
- The extracellular matrix and ciliary signaling.Current opinion in cell biology · 2012Review
- Article
- Axonemal positioning and orientation in three-dimensional space for primary cilia: what is known, what is assumed, and what needs clarification.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Review
- Effect of localization, length and orientation of chondrocytic primary cilium on murine growth plate organization.Journal of theoretical biology · 2011Article
- Primary cilia are highly oriented with respect to collagen direction and long axis of extensor tendon.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2010Article
- Cilia involvement in patterning and maintenance of the skeleton.Current topics in developmental biology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The chondrocytic primary cilium has been hypothesized to act as a mechano-sensor, analogously to primary cilium of cells in epithelial tissues. We hypothesize that mechanical inputs during growth, sensed through the primary cilium, result in directed secretion of the extracellular matrix, thereby establishing tissue anisotropy in growth plate cartilage. The cilium, through its orientation in three-dimensional space, is hypothesized to transmit to the chondrocyte the preferential direction for matrix secretion. This paper reports on the application of classical mathematical methods to develop an algorithm that addresses the particular challenges relative to the assessment of the orientation of the primary cilium in growth plate cartilage, based on image analysis of optical sections visualized by multiphoton microscopy. Specimens are prepared by rapid cold precipitation-based fixation to minimize possible artifactual post-mortem alterations of ciliary orientation. The ciliary axoneme is localized by immunocytochemistry with antibody acetylated-alpha-tubulin. The method is applicable to investigation of ciliary orientation in different zones of the growth plate, under either normal or altered biomechanical environments. The methodology is highly flexible and adaptable to other connective tissues where tissue anisotropy and directed secretion of extracellular matrix components are hypothesized to depend on the tissue's biomechanical environment during development and growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.