ArticleExperimental eye research2007
Transgenic overexpression of connexin50 induces cataracts.
Article in Experimental eye research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Loss of fiber cell communication may contribute to the development of cataracts of many different etiologies.Frontiers in physiology · 2022Review
- Association of Alpha-Crystallin with Fiber Cell Plasma Membrane of the Eye Lens Accompanied by Light Scattering and Cataract Formation.Membranes · 2021Review
- Degradation of connexin 50 protein causes waterclefts in human lens.Open medicine (Warsaw, Poland) · 2020Article
- New GJA8 variants and phenotypes highlight its critical role in a broad spectrum of eye anomalies.Human genetics · 2019Article
- N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis.Developmental biology · 2017Article
- Focus on lens connexins.BMC cell biology · 2017Review
- Human βA3/A1-crystallin splicing mutation causes cataracts by activating the unfolded protein response and inducing apoptosis in differentiating lens fiber cells.Biochimica et biophysica acta · 2016Article
- Lipid domains in intact fiber-cell plasma membranes isolated from cortical and nuclear regions of human eye lenses of donors from different age groups.Experimental eye research · 2015Article
- Molecular mechanism of formation of cortical opacity in CRYAAN101D transgenic mice.Investigative ophthalmology & visual science · 2014Article
- Connexin46fs380 causes progressive cataracts.Investigative ophthalmology & visual science · 2014Article
- Lipid-protein interactions in plasma membranes of fiber cells isolated from the human eye lens.Experimental eye research · 2014Article
- Connexin50D47A decreases levels of fiber cell connexins and impairs lens fiber cell differentiation.Investigative ophthalmology & visual science · 2013Article
- Connexin mutants and cataracts.Frontiers in pharmacology · 2013Article
- Properties of fiber cell plasma membranes isolated from the cortex and nucleus of the porcine eye lens.Experimental eye research · 2012Article
- The common modification in alphaA-crystallin in the lens, N101D, is associated with increased opacity in a mouse model.The Journal of biological chemistry · 2011Article
- Gap junctions or hemichannel-dependent and independent roles of connexins in cataractogenesis and lens development.Current molecular medicine · 2010Review
- Transgenic expression of AQP1 in the fiber cells of AQP0 knockout mouse: effects on lens transparency.Experimental eye research · 2010Article
- Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens.The Journal of cell biology · 2009Article
- Ultrastructural analysis of damage to nuclear fiber cell membranes in advanced age-related cataracts from India.Experimental eye research · 2008Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
To examine the effects of increased expression of Cx50 in the mouse lens, transgenic mice were generated using a DNA construct containing the human Cx50 coding region and a C-terminal FLAG epitope driven by the chicken betaB1-crystallin promoter. Expression of this protein in paired Xenopus oocytes induced gap junctional currents of similar magnitude to wild type human Cx50. Three lines of transgenic mice expressing the transgenic protein were analyzed. Lenses from transgenic mice were smaller than those from non-transgenic littermates, and had cataracts that were already visible at postnatal day 1. Expression of the transgene resulted in a 3- to 13-fold increase in Cx50 protein levels above those of non-transgenic animals. Light microscopy revealed alterations in epithelial cell differentiation, fiber cell structure, interactions between fiber cells and areas of liquefaction. Scanning electron microscopy showed fiber cells of varying widths with bulging areas along single fibers. Anti-Cx50 and anti-FLAG immunoreactivities were detected at appositional membranes and in intracellular vesicles in transgenic lenses. N-cadherin, Cx46, ZO-1 and aquaporin 0 localized mainly at the plasma membrane, although some N-cadherin and aquaporin 0 was associated with the intracellular vesicles. The abundance and solubility/integrity of alphaA-, alphaB-, beta- and gamma-crystallin were unaffected. These results demonstrate that transgenic expression of Cx50 in mice leads to cataracts associated with formation of cytoplasmic vesicles containing Cx50 and decreased or slowed epithelial differentiation without major alterations in the distribution of other integral membrane or membrane-associated proteins or the integrity/solubility of crystallins.
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