ArticleTransgenic research2007
Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- A single-cell screening platform accelerates functional genetics in plants.Nature biotechnology · 2026Article
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- APathogens (Basel, Switzerland) · 2025Article
- Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.).Plant biotechnology journal · 2025Article
- Hairpin inserts in viral genomes are stable when they conform to the thermodynamic properties of viral RNA substructures.Journal of virology · 2025Article
- Genome editing in cells of apple cultivar 'Fuji' using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components.Plant biotechnology (Tokyo, Japan) · 2024Article
- Plant-made pharmaceuticals.Plant biotechnology (Tokyo, Japan) · 2024Article
- Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves.BMC biotechnology · 2024Article
- Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.The plant genome · 2023Review
- Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2.Scientific reports · 2023Article
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Genome editing in rice mediated by miniature size Cas nuclease SpCas12f.Frontiers in genome editing · 2023Article
- A GoldenBraid-Compatible Virus-Based Vector System for Transient Expression of Heterologous Proteins in Plants.Viruses · 2022Article
- The Use of a Replicating Virus Vector ForFrontiers in bioengineering and biotechnology · 2022Article
- An EfficientFrontiers in plant science · 2021Article
- Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants.Plant & cell physiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the stability of expression constructs based on Potato virus X (PVX) as a function of insert length. Five different inserts ranging in length from 261 to 1,758 bp (human proinsulin, murine interleukin-10, HIV-1 nef, petunia expansin-1 and human gad65) were expressed using a PVX vector in Nicotiana benthamiana plants for three sequential passages. Using a competitive RT-PCR approach we demonstrated that insert-deletion could occur in the first infection cycle for all inserts, but that this was much more likely to be the case for longer ones. This suggested a negative correlation between insert length and vector stability. Sequence analysis of the deleted constructs suggested that recombination usually occurred at sites close to the duplicated sub-genomic promoter, but in a smaller number of cases the foreign gene itself was probably involved, resulting in partially deleted constructs containing transgene fragments. The implications of these results in the context of recombinant protein expression and its risks are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.