ArticleMarine biotechnology (New York, N.Y.)
Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Establishment and characterization of a continuous cell line from eye of the brown-marbled grouper (Epinephelus fuscoguttatus) for virological studies.In vitro cellular & developmental biology. Animal · 2026Article
- Effect of temperature on the replication of striped jack nervous necrosis virus in kidney cell line of rainbow trout (RTK).Virusdisease · 2025Article
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- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- Establishment and Characterization of Continuous Satellite Muscle Cells from Olive Flounder (Cells · 2023Article
- An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals.Fish physiology and biochemistry · 2022Article
- Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus.Scientific reports · 2020Article
- Assessment of fish iridoviruses using a novel cell line GS-1, derived from the spleen of orange-spotted grouper Epinephelus coioides (Hamilton) and susceptible to ranavirus and megalocytivirus.The Journal of veterinary medical science · 2018Article
- Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.Fish physiology and biochemistry · 2018Article
- JNK1 Derived from Orange-Spotted Grouper, Epinephelus coioides, Involving in the Evasion and Infection of Singapore Grouper Iridovirus (SGIV).Frontiers in microbiology · 2016Article
- Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India.In vitro cellular & developmental biology. Animal · 2015Article
- Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale.Fish physiology and biochemistry · 2015Article
- RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax.Cytotechnology · 2014Article
- Development and characterization of a new cell line CF from caudal fin of knifefish, Chitala chitala (Hamilton-Buchanan).In vitro cellular & developmental biology. Animal · 2013Article
- Establishment of testicular and ovarian cell lines from Honmoroko (Gnathopogon caerulescens).Fish physiology and biochemistry · 2013Article
- Betanodavirus of marine and freshwater fish: distribution, genomic organization, diagnosis and control measures.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).Fish physiology and biochemistry · 2012Article
- Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.).Fish physiology and biochemistry · 2012Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
- Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865).In vitro cellular & developmental biology. Animal · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two new cell lines, SIMH and SIGE, were derived from the heart of milkfish (Chanos chanos), a euryhaline teleost, and from the eye of grouper (Epinephelus coioides), respectively. These cell lines were maintained in Leibovitz's L-15 supplemented with 20% fetal bovine serum (FBS). The SIMH cell line was subcultured more than 50 times over a period of 210 days and SIGE cell line has been subcultured 100 times over a period of 1 1/2 years. The SIMH cell line consists predominantly of fibroblastic-like cells. The SIGE cell line consists predominantly of epithelial cells. Both the cell lines were able to grow at temperatures between 25 and 32 degrees C with an optimum temperature of 28 degrees C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 28 degrees C with optimum growth at the concentrations of 15% or 20% FBS. Seven marine fish viruses were tested to determine the susceptibility of these cell lines. The SIGE cell line was found to be susceptible to nodavirus, MABV NC-1 and Y6, and the infection was confirmed by cytopathic effect (CPE) and reverse transcriptase-polymerase chain reaction. When these cells were transfected with pEGFP-N1 vector DNA, significant fluorescent signals were observed, suggesting that these cell lines can be a useful tool for transgenic and genetic manipulation studies. Further, these cell lines are characterized by immunocytochemistry using confocal laser scanning microscopy (CFLSM).
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