ArticleAnalytical and bioanalytical chemistry2007
Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques.
Article in Analytical and bioanalytical chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- Artificial Intelligence-Driven Analysis of Antimicrobial-Resistant and Biofilm-Forming Pathogens on Biotic and Abiotic Surfaces.Antibiotics (Basel, Switzerland) · 2024Review
- Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy.Biofilm · 2023Article
- In situ monitoring of Lentilactobacillus parabuchneri biofilm formation via real-time infrared spectroscopy.NPJ biofilms and microbiomes · 2022Article
- A Technological Understanding of Biofilm Detection Techniques: A Review.Materials (Basel, Switzerland) · 2020Review
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- Bordetella Pertussis virulence factors in the continuing evolution of whooping cough vaccines for improved performance.Medical microbiology and immunology · 2018Review
- Hyperbiofilm Formation by Bordetella pertussis Strains Correlates with Enhanced Virulence Traits.Infection and immunity · 2017Article
- The Bordetella Bps Polysaccharide Is Required for Biofilm Formation and Enhances Survival in the Lower Respiratory Tract of Swine.Infection and immunity · 2017Article
- Bordetella adenylate cyclase toxin interacts with filamentous haemagglutinin to inhibit biofilm formation in vitro.Molecular microbiology · 2017Article
- BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii.PloS one · 2016Article
- Optical Sensing of Microbial Life on Surfaces.Applied and environmental microbiology · 2015Review
- Bordetella pertussis Isolates from Argentinean Whooping Cough Patients Display Enhanced Biofilm Formation Capacity Compared to Tohama I Reference Strain.Frontiers in microbiology · 2015Article
- Biofilm-specific extracellular matrix proteins of nontypeable Haemophilus influenzae.Pathogens and disease · 2014Article
- Deciphering host genotype-specific impacts on the metabolic fingerprint of Listeria monocytogenes by FTIR spectroscopy.PloS one · 2014Article
- Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis.PloS one · 2014Article
- Experimental approaches to investigating the vaginal biofilm microbiome.Methods in molecular biology (Clifton, N.J.) · 2014Article
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- The Bps polysaccharide of Bordetella pertussis promotes colonization and biofilm formation in the nose by functioning as an adhesin.Molecular microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
This work describes the application of several analytical techniques to characterize the development of Bordetella pertussis biofilms and to examine, in particular, the contribution of virulence factors in this development. Growth of surface-attached virulent and avirulent B. pertussis strains was monitored in continuous-flow chambers by techniques such as the crystal violet method, and nondestructive methodologies like fluorescence microscopy and Fourier transform (FT) IR spectroscopy. Additionally, B. pertussis virulent and avirulent strains expressing green fluorescent protein were grown adhered to the base of a glass chamber of 1-microm thickness. Three-dimensional images of mature biofilms, acquired by confocal laser scanning microscopy, were quantitatively analysed by means of the computer program COMSTAT. Our results indicate that only the virulent (Bvg(+)) phase of B. pertussis is able to attach to surfaces and develop a mature biofilm. In the virulent phase these bacteria are capable of producing a biofilm consisting of microcolonies of approximately 200 microm in diameter and 24 microm in depth. FTIR spectroscopy allowed us not only to follow the dynamics of biofilm growth through specific biomass and biofilm marker absorption bands, but also to monitor the maturation of the biofilm by means of the increase of the carbohydrate-to-protein ratio.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.