ArticleJournal of clinical microbiology2007
Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 65 citations in OpenAlex.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.PloS one · 2012Trial
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- Congenital Sepsis withMicroorganisms · 2024Review
- Performance of a Real-Time PCR Assay for the Detection of FiveJournal of fungi (Basel, Switzerland) · 2023Article
- Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.Journal of clinical laboratory analysis · 2022Article
- Ear microbiota and middle ear disease: a longitudinal pilot study of Aboriginal children in a remote south Australian setting.BMC microbiology · 2022Article
- Article
- Article
- Ureaplasma parvum genotype, combined vaginal colonisation with Candida albicans, and spontaneous preterm birth in an Australian cohort of pregnant women.BMC pregnancy and childbirth · 2016Article
- A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water.BMC biotechnology · 2015Article
- Differentiation and phylogenetic relationships in Mycobacterium spp with special reference to the RNase P RNA gene rnpB.Current microbiology · 2014Article
- Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep.Pediatric research · 2014Article
- Diagnostics method for the rapid quantitative detection and identification of low-level contamination of high-purity water with pathogenic bacteria.Journal of industrial microbiology & biotechnology · 2013Article
- Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry.Journal of clinical microbiology · 2013Article
- Candida infections and their prevention.ISRN preventive medicine · 2013Review
- The first Korean case of candidemia due to Candida dubliniensis.Annals of laboratory medicine · 2012Article
- Evaluation of Luminex xTAG fungal analyte-specific reagents for rapid identification of clinically relevant fungi.Journal of clinical microbiology · 2011Article
- Analysis of performance of a PCR-based assay to detect DNA of Aspergillus fumigatus in whole blood and serum: a comparative study with clinical samples.Journal of clinical microbiology · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a single-tube multiplex real-time PCR method for the detection of the eight most common Candida species causing septicemia: Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis. The method developed targets the RNase P RNA gene RPR1. Sequences of this gene were determined for seven of the Candida species and showed surprisingly large sequence variation. C. glabrata was found to have a gene that was five times longer gene than those of the other species, and the nucleotide sequence similarity between C. krusei and C. albicans was as low as 55%. The multiplex PCR contained three probes that enabled the specific detection of C. albicans, C. glabrata, and C. krusei and a fourth probe that allowed the general detection of the remaining species. The method was able to detect 1 to 10 genome copies when the detection limit was tested repeatedly for the four species C. albicans, C. glabrata, C. krusei, and C. guilliermondii. No significant difference in the detection limit was seen when the multiplex format was compared with single-species PCR, i.e., two primers and one probe. The method detected eight clinically relevant Candida species and did not react with other tested non-Candida species or human DNA. The assay was applied to 20 blood samples from nine patients and showed a sensitivity similar to that of culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.