Evidence map›Paper›PMID 17215333›Full record

ArticleClinical and vaccine immunology : CVI2007

Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale.

N I Strik, A R Alleman, A F Barbet, H L Sorenson, H L Wamsley, F P Gaschen, N Luckschander, S Wong, F Chu, J E Foley and 3 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and vaccine immunology : CVI, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Review
  4. Article
  5. Article
  6. Article
  7. Presence ofAnimals : an open access journal from MDPI · 2023
    Article
  8. Unravelling the diversity ofCurrent research in microbial sciences · 2023
    Article
  9. Article
  10. Article
  11. Comparison of three nucleic acid-based tests for detectingThe Onderstepoort journal of veterinary research · 2017
    Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Characterization of recombinant MSP5 Anaplasma marginale Havana isolate.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2009
    Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

N I StrikCollege of Veterinary Medicine, University of Florida, 2015 S.W. 16th Ave., Box 100103, Gainesville, FL 32610, USA.
A R Alleman
A F Barbet
H L Sorenson
H L Wamsley
F P Gaschen
N Luckschander
S Wong
F Chu
J E Foley
A Bjoersdorff
S Stuen
D P Knowles
University of Bern · CHUniversity of California, Davis · USNew York State Department of Health · USUniversity of Florida · USLandstinget i Kalmar län · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Major surface protein 5 (Msp5) of Anaplasma marginale is highly conserved in the genus Anaplasma and the antigen used in a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for serologic identification of cattle with anaplasmosis. This study analyzes the degrees of conservation of Msp5 among various isolates of Anaplasma phagocytophilum and the extent of serologic cross-reactivity between recombinant Msp5 (rMsp5) of Anaplasma marginale and A. phagocytophilum. The msp5 genes from various isolates of A. phagocytophilum were sequenced and compared. rMsp5 proteins of A. phagocytophilum and A. marginale were used separately in an indirect ELISA to detect cross-reactivity in serum samples from humans and dogs infected with A. phagocytophilum and cattle infected with A. marginale. Serum samples were also tested with a commercially available competitive ELISA that uses monoclonal antibody ANAF16C1. There were 100% sequence identities in the msp5 genes among all of the A. phagocytophilum isolates from the United States and a horse isolate from Sweden. Sheep isolates from Norway and dog isolates from Sweden were 99% identical to one another but differed in 17 base pairs from the United States isolates and the horse isolate. Serologic cross-reactivity was identified when serum samples from cattle infected with A. marginale were reacted with rMsp5 of A. phagocytophilum and when serum samples from humans and dogs infected with A. phagocytophilum were reacted with rMsp5 of A. marginale in an indirect-ELISA format. Serum samples from dogs or humans infected with A. phagocytophilum did not cross-react with rMsp5 of A. marginale when tested with the commercially available cELISA. These results suggest that rMsp5 of A. phagocytophilum is highly conserved among United States and European isolates and that serologic distinction between A. phagocytophilum and A. marginale infections cannot be accomplished if rMsp5 from either organism is used in an indirect ELISA.

Indexed as

Anaplasma marginaleAnaplasma phagocytophilumAnimalsBacterial Outer Membrane ProteinsBase SequenceCattleDogsEnzyme-Linked Immunosorbent AssayHorsesHumansMolecular Sequence DataSheepBacterial Outer Membrane Proteinsmajor surface protein 5, Anaplasma

Identifiers

PMID17215333
PMCPMC1828860
OpenAlexW2103438052

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.