ArticleBMC developmental biology2007
Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation.
Article in BMC developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 54 citations in OpenAlex.
- Mitogen-activated protein kinase kinase 4 (MKK4) as a promising therapeutic target in liver diseases: a review.Journal of enzyme inhibition and medicinal chemistry · 2026Review
- Hyperosmotic stress induces 2-cell-like cells through ROS and ATR signaling.EMBO reports · 2023Article
- The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes.Animals : an open access journal from MDPI · 2022Article
- Overexpression of MAP3K3 promotes tumour growth through activation of the NF-κB signalling pathway in ovarian carcinoma.Scientific reports · 2019Article
- Vis-à-vis: a focus on genetic features of cerebral cavernous malformations and brain arteriovenous malformations pathogenesis.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2019Review
- Association of single-nucleotide polymorphisms inArchives animal breeding · 2018Article
- Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.Molecular human reproduction · 2017Article
- Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.Scientific reports · 2016Article
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- Loss of genomic imprinting in mouse embryos with fast rates of preimplantation development in culture.Biology of reproduction · 2012Article
- Article
- PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.The Journal of biological chemistry · 2010Article
- Cerebral cavernous malformations proteins inhibit Rho kinase to stabilize vascular integrity.The Journal of experimental medicine · 2010Article
- A major effect of simulated microgravity on several stages of preimplantation mouse development is lethality associated with elevated phosphorylated SAPK/JNK.Reproductive sciences (Thousand Oaks, Calif.) · 2009Article
- Genomic RNA profiling and the programme controlling preimplantation mammalian development.Molecular human reproduction · 2008Article
- Increased susceptibility to collagen-induced arthritis in female mice carrying congenic Cia40/Pregq2 fragments.Arthritis research & therapy · 2008Article
- The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro.BMC developmental biology · 2007Article
- Quantitative tomography of mouse early embryo.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMechanisms that confer an ability to respond positively to environmental osmolarity are fundamental to ensuring embryo survival during the preimplantation period. Activation of p38 mitogen-activated protein kinase (MAPK) occurs following exposure to hyperosmotic treatment. Recently, a novel scaffolding protein called Osmosensing Scaffold for MEKK3 (OSM) was linked to p38 MAPK activation in response to sorbitol-induced hypertonicity. The human ortholog of OSM is cerebral cavernous malformation 2 (CCM2). The present study was conducted to investigate whether CCM2 is expressed during mouse preimplantation development and to determine whether this scaffolding protein is associated with p38 MAPK activation following exposure of preimplantation embryos to hyperosmotic environments.
resultsOur results indicate that Ccm2 along with upstream p38 MAPK pathway constituents (Map3k3, Map2k3, Map2k6, and Map2k4) are expressed throughout mouse preimplantation development. CCM2, MAP3K3 and the phosphorylated forms of MAP2K3/MAP2K6 and MAP2K4 were also detected throughout preimplantation development. Embryo culture in hyperosmotic media increased p38 MAPK activity in conjunction with elevated CCM2 levels.
conclusionThese results define the expression of upstream activators of p38 MAPK during preimplantation development and indicate that embryo responses to hyperosmotic environments include elevation of CCM2 and activation of p38 MAPK.
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